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Literature summary extracted from

  • Veret, J.; Coant, N.; Berdyshev, E.V.; Skobeleva, A.; Therville, N.; Bailbe, D.; Gorshkova, I.; Natarajan, V.; Portha, B.; Le Stunff, H.
    Ceramide synthase 4 and de novo production of ceramides with specific N-acyl chain lengths are involved in glucolipotoxicity-induced apoptosis of INS-1 beta-cells (2011), Biochem. J., 438, 177-189 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.24 gene CERS4, quantitative real-time PCR enzyme expression analysis, transient overexpression of HA-tagged CerS4 in INS-1 cells increasing the levels of ceramides C18:0 and C22:0 by 20%, but it has no effect on the accumulation of ceramides C16:0 and C24:0 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.24 sphingosine + behenoyl-CoA Rattus norvegicus
-
 N-behenoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + stearoyl-CoA Rattus norvegicus
-
 N-stearoylsphingosine + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Rattus norvegicus D3ZU86
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 INS-1 cell
-
 Rattus norvegicus
-
2.3.1.24 insulinoma cell
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 sphingosine + behenoyl-CoA
-
 Rattus norvegicus N-behenoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + stearoyl-CoA
-
 Rattus norvegicus N-stearoylsphingosine + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 ceramide synthase 4
-
 Rattus norvegicus
2.3.1.24 CerS4
-
 Rattus norvegicus

Expression

EC Number Organism Comment Expression
2.3.1.24 Rattus norvegicus palmitate increases the levels of CerS4 mRNA 3fold in the presence of 30 mM glucose, overview up

General Information

EC Number General Information Comment Organism
2.3.1.24 physiological function CerS4 plays a critical role in beta-cells apoptosis induced by glucolipotoxicity through the synthesis of specific ceramide species such as C18:0. Glucolipotoxicity activates caspase 3/7 through ceramide accumulation in INS-1 cells. Transient overexpression of HA-tagged CerS4 increases the levels of ceramides C18:0 and C22:0 by 20%, but has no effect on the accumulation of ceramides C16:0 and C24:0. CerS4 potentiates palmitate-induced beta-cell apoptosis. Overexpression of HA-tagged CerS4 potentiates ceramide accumulation induced by stearate, which is particularly toxic for beta-cells Rattus norvegicus