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Literature summary extracted from

  • Mizutani, Y.; Kihara, A.; Igarashi, Y.
    LASS3 (longevity assurance homologue 3) is a mainly testis-specific (dihydro)ceramide synthase with relatively broad substrate specificity (2006), Biochem. J., 398, 531-538 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.297 gene CERS3, DNA and amino acid sequence determination and analysis, cloning of two transcriptional variants of LASS3 cDNA, which encode a 384-amino-acid protein (LASS3) and a 419-amino-acid protein (LASS3-long), reverse transcription (RT)-PCR expression analysis, recombinant expression in HEK-293T cells Mus musculus
2.3.1.298 expressed in HEK-293T cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.297 fumonisin B1
-
 Mus musculus
2.3.1.298 fumonisin B1
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.297 membrane LASS3 has up to six transmembrane spanning domains Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.297 sphinganine + arachidoyl-CoA Mus musculus
-
 N-arachidoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + behenoyl-CoA Mus musculus
-
 N-behenoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + cerotoyl-CoA Mus musculus
-
 N-cerotoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + lignoceroyl-CoA Mus musculus
-
 N-lignoceroylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + palmitoyl-CoA Mus musculus
-
 N-palmitoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + stearoyl-CoA Mus musculus
-
 N-stearoylsphinganine + CoA
-
 ?
2.3.1.298 fatty acyl-CoA + dihydrosphingosine Mus musculus
-
 ceramide + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.297 Mus musculus Q1A3B0
-
-
2.3.1.298 Mus musculus Q1A3B0
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.297 no glycoprotein LASS3 has no putative N-glycosylation site Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.297 testis Lass3 is mainly testis-specific Mus musculus
-
2.3.1.298 testis enzyme expression is limited almost solely to testis Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.297 additional information enzyme LASS3 shows a relatively broad substrate specificity. Both LASS3 isoforms increase the production of several ceramide species, including C18:0- and C24:0-ceramides. LASS3 exhibits relatively low substrate specificity toward fatty acyl-CoAs. Lysates prepared from LASS3-overproducing cells show significant activity toward several fatty acyl-CoAs, including C18:0-, C22:0-, and C24:0-CoA, with the highest activity for C18:0. C16:0-, C20:0-, and C26:0-CoA are also used as substrates by LASS3, albeit with low activity. LASS3-overproducing cells synthesize ceramide from C18:0-CoA with the highest activity at all concentrations tested. This in vitro result is quite consistent with the in vivo results Mus musculus ?
-
 ?
2.3.1.297 sphinganine + arachidoyl-CoA
-
 Mus musculus N-arachidoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + arachidoyl-CoA low activity Mus musculus N-arachidoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + behenoyl-CoA
-
 Mus musculus N-behenoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + behenoyl-CoA moderate activity Mus musculus N-behenoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + cerotoyl-CoA
-
 Mus musculus N-cerotoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + cerotoyl-CoA low activity Mus musculus N-cerotoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + lignoceroyl-CoA
-
 Mus musculus N-lignoceroylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + lignoceroyl-CoA high activity Mus musculus N-lignoceroylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + palmitoyl-CoA
-
 Mus musculus N-palmitoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + palmitoyl-CoA low activity Mus musculus N-palmitoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + stearoyl-CoA
-
 Mus musculus N-stearoylsphinganine + CoA
-
 ?
2.3.1.297 sphinganine + stearoyl-CoA preferred substrate Mus musculus N-stearoylsphinganine + CoA
-
 ?
2.3.1.298 arachidoyl-CoA + dihydrosphingosine weak activity Mus musculus CoA + N-arachidoyl-dihydrosphingosine
-
 ?
2.3.1.298 behenoyl-CoA + dihydrosphingosine
-
 Mus musculus CoA + N-behenoyldihydrosphingosine
-
 ?
2.3.1.298 cerotoyl-CoA + dihydrosphingosine weak activity Mus musculus CoA + N-cerotoyl-dihydrosphingosine
-
 ?
2.3.1.298 fatty acyl-CoA + dihydrosphingosine
-
 Mus musculus ceramide + CoA
-
 ?
2.3.1.298 lignoceroyl-CoA + dihydrosphingosine
-
 Mus musculus CoA + N-lignoceroyldihydrosphingosine
-
 ?
2.3.1.298 additional information the enzyme exhibits relatively low substrate specificity toward fatty-acyl CoAs Mus musculus ?
-
 ?
2.3.1.298 palmitoyl-CoA + dihydrosphingosine high activity Mus musculus CoA + N-palmitoyldihydrosphingosine
-
 ?
2.3.1.298 stearoyl-CoA + dihydrosphingosine highest activity Mus musculus CoA + N-stearoyldihydrosphingosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.297 ? x * 46100, about, isozyme LASS3, sequence calculation, x * 50000, about, isozyme LASS3-long, sequence calculation Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.297 (dihydro)ceramide synthase
-
 Mus musculus
2.3.1.297 ceramide synthase 3
-
 Mus musculus
2.3.1.297 CerS3
-
 Mus musculus
2.3.1.297 LASS3
-
 Mus musculus
2.3.1.297 longevity assurance homologue 3
-
 Mus musculus
2.3.1.298 (dihydro)ceramide synthase
-
 Mus musculus
2.3.1.298 LASS3 gene name, longevity assurance homologue 3 Mus musculus
2.3.1.298 LASS3-long transcriptional variant Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.297 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.297 7.5
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.297 additional information two transcriptional variants of mouse LASS3 cDNA encoding the LASS3 isoforms, which differ in transcriptional initiation sites and polypeptides length, LASS3 and LASS3-long Mus musculus
2.3.1.297 physiological function overproduction of LASS3 causes increased levels of C18:0-, C22:0-, and C24:0-ceramides Mus musculus