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Literature summary extracted from

  • Mizutani, Y.; Kihara, A.; Igarashi, Y.
    Mammalian Lass6 and its related family members regulate synthesis of specific ceramides (2005), Biochem. J., 390, 263-271 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.24 DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells Mus musculus
2.3.1.24 DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells, and recombinant expression of C-terminally GFP-tagged Lass6 Mus musculus
2.3.1.291 expression in HEK 293T cell Mus musculus
2.3.1.297 DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells Mus musculus
2.3.1.299 DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.24 additional information no loss of activity is observed for the unglycosylated mutant (HA-tagged Lass6-N18Q) when compared with either the glycosylated mutant HA-tagged Lass6-N285Q or the glycosylated wild-type Lass6 Mus musculus
2.3.1.24 N18Q site-directed mutagenesis, a glycosylation site mutant Mus musculus
2.3.1.24 N285Q site-directed mutagenesis, a putative glycosylation site mutant Mus musculus
2.3.1.297 additional information Lass4 overproduction causing increases in both middle- and long-chain ceramides Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.24 endoplasmic reticulum membrane the C-terminus of Lass6 is exposed to the cytosolic side of the endoplasmic reticulum membrane Mus musculus 5789
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.291 additional information Mus musculus isoform LASS5 preferentially produces C14:0 and C16:0 ceramides ?
-
 ?
2.3.1.291 palmitoyl-CoA + a sphingoid base Mus musculus
-
 an N-(palmitoyl)-sphingoid base + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Mus musculus Q8C172
-
-
2.3.1.24 Mus musculus Q9D6K9
-
-
2.3.1.291 Mus musculus Q9D6K9
-
-
2.3.1.297 Mus musculus Q924Z4
-
-
2.3.1.297 Mus musculus Q9D6J1
-
-
2.3.1.299 Mus musculus P27545
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.24 glycoprotein Asn18, but not Asn285, of Lass6 is glycosylated, deglycosylation of Lass6 with PNGase F (N-glycosidase F). Lass6 is modified by N-glycosylation of a high-mannose and/or hybrid type. N-glycosylation is not essential for the dihydroceramide synthase activity of Lass6 Mus musculus
2.3.1.24 glycoprotein the N-glycosylation of Lass5 appears as two bands, deglycosylation of Lass5 with PNGase F (N-glycosidase F) Mus musculus
2.3.1.291 glycoprotein protein is N-glycosylated Mus musculus
2.3.1.297 glycoprotein the N-glycosylation of Lass2 appears as three bands, deglycosylation of Lass2 with PNGase F (N-glycosidase F) Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.24 brain
-
 Mus musculus
-
2.3.1.24 kidney
-
 Mus musculus
-
2.3.1.24 kidney high expression level Mus musculus
-
2.3.1.24 additional information isozymes mRNAs for Lass2, Lass4, Lass5 and Lass6 are found to be mostly ubiquitous, although no expression is detected in muscle for any of these. No expression of Lass6 is observed in heart, muscle, spleen or stomach. Lass6 is most highly expressed in kidney, followed by brain Mus musculus
-
2.3.1.24 additional information isozymes mRNAs for Lass2, Lass4, Lass5 and Lass6 are found to be mostly ubiquitous, although no expression is detected in muscle for any of these. The highest expression of Lass5 is in testis, and the expression in kidney is also high Mus musculus
-
2.3.1.24 testis high expression level Mus musculus
-
2.3.1.291 kidney high expression level Mus musculus
-
2.3.1.291 testis highest expression level Mus musculus
-
2.3.1.297 kidney high expression level Mus musculus
-
2.3.1.297 liver high expression level Mus musculus
-
2.3.1.297 additional information isozymes mRNAs for Lass2, Lass4, Lass5 and Lass6 are found to be mostly ubiquitous, although no expression is detected in muscle for any of these. Lass2 shows the highest expression in liver, but also high expression in kidney Mus musculus
-
2.3.1.297 additional information isozymes mRNAs for Lass2, Lass4, Lass5 and Lass6 are found to be mostly ubiquitous, although no expression is detected in muscle for any of these. Lass4 is strongly expressed in skin, with no apparent expression in muscle, testis, or thymus Mus musculus
-
2.3.1.297 skin high expression level Mus musculus
-
2.3.1.299 brain high expression level Mus musculus
-
2.3.1.299 additional information Lass1 is detected in brain and just weakly in muscle, indicating that expression is mostly brain-specific Mus musculus
-
2.3.1.299 muscle low expression level Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 additional information both Lass5- and Lass6-overproducing cells exhibit high dihydroceramide synthesis activity using C16:0- and C14:0-CoAs and low activity with C12:0- and C18:0-CoAs. On the other hand, whereas the Lass5-overproducing cells demonstrates significant C18:1-ceramide synthesis, the Lass6-overproducing cells do not show any such increase. Recombinant Lass6 produces shorter ceramide species (C14:0- and C16:0-ceramides) in transgenic HEK-293T cells Mus musculus ?
-
 ?
2.3.1.24 additional information both Lass5- and Lass6-overproducing cells exhibit high dihydroceramide synthesis activity using C16:0- and C14:0-CoAs and low activity with C12:0- and C18:0-CoAs. On the other hand, whereas the Lass5-overproducing cells demonstrates significant C18:1-ceramide synthesis, the Lass6-overproducing cells do not show any such increase. The recombinant Lass5 shows a preference for C16 substrates in transgenic HEK-293T cells Mus musculus ?
-
 ?
2.3.1.24 sphingosine + lauroyl-CoA low activity Mus musculus N-lauroylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + myristoyl-CoA
-
 Mus musculus N-myristoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + oleoyl-CoA low activity Mus musculus N-oleoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + palmitoyl-CoA
-
 Mus musculus N-palmitoylsphingosine + CoA
-
 ?
2.3.1.24 sphingosine + stearoyl-CoA low activity Mus musculus N-stearoylsphingosine + CoA
-
 ?
2.3.1.291 additional information isoform LASS5 preferentially produces C14:0 and C16:0 ceramides Mus musculus ?
-
 ?
2.3.1.291 palmitoyl-CoA + a sphingoid base
-
 Mus musculus an N-(palmitoyl)-sphingoid base + CoA
-
 ?
2.3.1.297 additional information both C22:0- and C24:0-CoAs are used effectively for Lass2- and Lass4-dependent ceramide synthesis, and C26:0-, C20:0-, and C18:0-CoAs also act as substrates, albeit weakly. Recombinant Lass2 produces longer ceramides such as C22:0 ceramides and C24:0 ceramides in transgenic HEK-293T cells Mus musculus ?
-
 ?
2.3.1.297 additional information both C22:0- and C24:0-CoAs are used effectively for Lass2- and Lass4-dependent ceramide synthesis, and C26:0-, C20:0-, and C18:0-CoAs also act as substrates, albeit weakly. Recombinant Lass4 produces longer ceramides such as C22:0 and C24:0 ceramides in transgenic HEK-293T cells Mus musculus ?
-
 ?
2.3.1.297 sphingosine + arachidoyl-CoA low activity Mus musculus N-arachidoylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + behenoyl-CoA
-
 Mus musculus N-behenoylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + hexacosanoyl-CoA low activity Mus musculus N-hexacosanoylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + lignoceroyl-CoA
-
 Mus musculus N-lignoceroylsphingosine + CoA
-
 ?
2.3.1.297 sphingosine + stearoyl-CoA low activity Mus musculus N-stearoylsphingosine + CoA
-
 ?
2.3.1.299 additional information C18:0-CoA is the best substrate for Lass1-dependent ceramide synthesis. C18:1-CoA, which has the same chain length as C18:0, is not a good substrate at all. Recombinant Lass1 produces preferentially C18:0-ceramides in transgenic HEK-293T cells Mus musculus ?
-
 ?
2.3.1.299 sphingosine + stearoyl-CoA
-
 Mus musculus N-stearoylsphingosine + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.24 ? x * 44800, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 LASS5
-
 Mus musculus
2.3.1.24 Lass6
-
 Mus musculus
2.3.1.24 trh1-like
-
 Mus musculus
2.3.1.24 trh4
-
 Mus musculus
2.3.1.291 CerS5
-
 Mus musculus
2.3.1.291 LASS5
-
 Mus musculus
2.3.1.297 ceramide synthase 2
-
 Mus musculus
2.3.1.297 ceramide synthase 4
-
 Mus musculus
2.3.1.297 CerS2
-
 Mus musculus
2.3.1.297 CerS4
-
 Mus musculus
2.3.1.297 Lass2
-
 Mus musculus
2.3.1.297 Lass4
-
 Mus musculus
2.3.1.297 trh1
-
 Mus musculus
2.3.1.297 trh3
-
 Mus musculus
2.3.1.299 ceramide synthase 1
-
 Mus musculus
2.3.1.299 CerS1
-
 Mus musculus
2.3.1.299 LASS1
-
 Mus musculus
2.3.1.299 UOG1
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.24 37
-
 assay at Mus musculus
2.3.1.297 37
-
 assay at Mus musculus
2.3.1.299 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7.5
-
 assay at Mus musculus
2.3.1.297 7.5
-
 assay at Mus musculus
2.3.1.299 7.5
-
 assay at Mus musculus

General Information

EC Number General Information Comment Organism
2.3.1.24 evolution Lass proteins are known to contain a TLC [TRAM/Lag1p/ CLN8 (ceroid-lipofuscinoses, neuronal 8)] homology domain with the Lag1 motif. Lass family members Lass2, Lass4 and Lass5, but not Lass1, also contain a HOX (homeobox) domain Mus musculus
2.3.1.24 evolution murine Lass6 is a member of the mouse Lass family. It exhibits the highest identity with Lass5 (61.7% identity and 68.2% similarity) and the lowest identity with Lass1 (16.0% identity and 27.4%similarity). Lass proteins are known to contain a TLC [TRAM/Lag1p/ CLN8 (ceroid-lipofuscinoses, neuronal 8)] homology domain with the Lag1 motif. Lass family members Lass2, Lass4 and Lass5, but not Lass1, also contain a HOX (homeobox) domain Mus musculus
2.3.1.24 additional information the N-terminus is essential for catalytic activity Mus musculus
2.3.1.297 evolution Lass proteins are known to contain a TLC [TRAM/Lag1p/CLN8 (ceroid-lipofuscinoses, neuronal 8)] homology domain with the Lag1 motif. Lass family members Lass2, Lass4 and Lass5, but not Lass1, also contain a HOX (homeobox) domain Mus musculus
2.3.1.297 malfunction Lass4 overproduction caused increases in both middle- and long-chain ceramides Mus musculus
2.3.1.297 additional information the N-terminus is essential for catalytic activity Mus musculus
2.3.1.299 evolution Lass proteins are known to contain a TLC [TRAM/Lag1p/CLN8 (ceroid-lipofuscinoses, neuronal 8)] homology domain with the Lag1 motif. Lass family members Lass2, Lass4 and Lass5, but not Lass1, also contain a HOX (homeobox) domain Mus musculus
2.3.1.299 additional information the N-terminus is essential for catalytic activity Mus musculus