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Literature summary extracted from

  • Kong, W.; Shiota, S.; Shi, Y.; Nakayama, H.; Nakayama, K.
    A novel peroxiredoxin of the plant Sedum lineare is a homologue of Escherichia coli bacterioferritin co-migratory protein Bcp (2000), Biochem. J., 351, 107-114 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expression in Escherichia coli overexpression system (BL21 DE3)pKD601. Expression of PrxQ suppresses the hypersensitivity of an Escherichia coli bcp mutant to peroxides, indicating that it might exert an antioxidant activity in vivo Sedum lineare

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.24 C44S expression of PrxQ suppresses the hypersensitivity of an Escherichia coli bcp mutant to peroxides, indicating that it might exert an antioxidant activity in vivo. Escherichia coli bcp cells producing the mutant enzyme show the same sensitivities to the organic peroxides as those of the control bcp cells Sedum lineare
1.11.1.24 C49S expression of PrxQ suppresses the hypersensitivity of an Escherichia coli bcp mutant to peroxides, indicating that it might exert an antioxidant activity in vivo. Escherichia coli bcp cells producing the mutant enzyme show the same sensitivities to the organic peroxides as those of the control bcp cells. Except that the bcp cells producing C49S show partial resistance to tert-butyl hydroperoxide Sedum lineare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 16699
-
 calculated from sequence Sedum lineare
1.11.1.24 17000
-
 SDS-PAGE Sedum lineare

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Sedum lineare Q9MB35
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.24 wild-type and mutant enzymes C44S and C49S Sedum lineare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.24 stem leaf
-
 Sedum lineare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 thioredoxin + cumene hydroperoxide
-
 Sedum lineare thioredoxin disulfide + H2O + cumene hydroxide
-
 ?
1.11.1.24 thioredoxin + H2O2
-
 Sedum lineare thioredoxin disulfide + 2 H2O
-
 ?
1.11.1.24 thioredoxin + tert-butyl hydroperoxide
-
 Sedum lineare thioredoxin disulfide + H2O + tert-butyl alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 monomer 1 * 17000, SDS-PAGE, the enzyme does not form a homodimer under non-reducing conditions, even after treatment with H2O2 Sedum lineare

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 PrxQ
-
 Sedum lineare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.24 Sedum lineare calculated from sequence
-
 9.7