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Literature summary extracted from

  • Hiroyama, M.; Takenawa, T.
    Purification and characterization of a lysophosphatidic acid-specific phosphatase (1998), Biochem. J., 336 ( Pt 2), 483-489 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.106 CHAPS activity is completely dependent on the presence of detergents such as Triton X-100, CHAPS, cholate and octyl-beta-glucoside Bos taurus
3.1.3.106 cholate activity is completely dependent on the presence of detergents such as Triton X-100, CHAPS, cholate and octyl-beta-glucoside Bos taurus
3.1.3.106 octylgucoside activity is completely dependent on the presence of detergents such as Triton X-100, CHAPS, cholate and octyl-beta-glucoside Bos taurus
3.1.3.106 Triton X-100 activity is completely dependent on the presence of detergents such as Triton X-100, CHAPS, cholate and octyl-beta-glucoside Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.106 Ca2+ 5 mM, about 90% inhibition Bos taurus
3.1.3.106 EDTA 5 mM, about 50% inhibition Bos taurus
3.1.3.106 Mg2+ 5 mM, about 50% inhibition Bos taurus
3.1.3.106 Mn2+ 5 mM, about 90% inhibition Bos taurus
3.1.3.106 N-ethylmaleimide 2.5 mM, 50% inhibition Bos taurus
3.1.3.106 NaF 2.5 mM, 85% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.106 0.038
-
 1-oleoyl-sn-glycerol 3-phosphate pH 7.5, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.106 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.106 additional information presence of Mg2+ is not required Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.106 1-oleoyl-sn-glycerol 3-phosphate + H2O Bos taurus
-
 1-oleoyl-sn-glycerol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.106 Bos taurus A6H757
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.106
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.106 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.106 1.1
-
 substrate oleoyl-sn-glycerol 3-phosphate, in Triton X-100 mixed micelles, pH 7.5, 37°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.106 1-oleoyl-sn-glycerol 3-phosphate + H2O
-
 Bos taurus 1-oleoyl-sn-glycerol + phosphate
-
 ?
3.1.3.106 additional information no substrates: cardiolipin, tetraoleoylbisphosphatidic acid, ceramide 1-phosphate or sphingosine 1-phosphate. The enzyme shows a slight activity towards phosphatidic acid (dioleolyl) and p-nitrophenyl phosphate in the neutral pH range around 7.5 and in the acidic pH range around 5.5, respectively Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.106 ? x * 44000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.106 Acp6
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.106 7.5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.106 6 8.5
-
 Bos taurus