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Literature summary extracted from

  • Cadel, S.; Darmon, C.; Desert, A.; Mahbouli, M.; Piesse, C.; Ghelis, T.; Lafont, R.; Foulon, T.
    The effects of curcumin, mangiferin, resveratrol and other natural plant products on aminopeptidase B activity (2019), Biochem. Biophys. Res. Commun., 512, 832-837 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.6 expressed in baculovirus infected SF9 insect cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.6 curcumin non-competitive inhibitor Rattus norvegicus
3.4.11.6 mangiferin mixed non-competitive inhibitor Rattus norvegicus
3.4.11.6 additional information At 0.2 mM, no significant inhibitory effect is observed with caffeic, chlorogenic, ferulic, salicylic and sinapic acids as well as with resveratrol Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.6 0.048
-
 L-Arg-7-amido-4-methylcoumarin at pH 7.4 and 28°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.6 Zn2+ contains zinc Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.6 His-Trap column chromatography and Superdex S200 gel filtration Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.6 L-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus L-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.11.6 L-Arg-L-Tyr-Gly-Gly-L-Phe-L-Leu + H2O
-
 Rattus norvegicus L-Arg + L-Tyr-Gly-Gly-L-Phe-L-Leu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.6 Ap-B
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.6 43
-
 L-Arg-7-amido-4-methylcoumarin at pH 7.4 and 28°C Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.6 0.046
-
 curcumin at pH 7.4 and 28°C Rattus norvegicus
3.4.11.6 0.105
-
 mangiferin at pH 7.4 and 28°C Rattus norvegicus