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Literature summary extracted from

  • Karthik, N.; Binod, P.; Pandey, A.
    SSF production, purification and characterization of chitin deacetylase from Aspergillus flavus (2018), Biocatal. Biotransform., 36, 296-306 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.41 Triton X-100 slight activation at 1% Aspergillus flavus
3.5.1.41 Tween 20 slight activation at 1% Aspergillus flavus
3.5.1.41 Tween 40 slight activation at 1% Aspergillus flavus
3.5.1.41 Tween 60 slight activation at 1% Aspergillus flavus
3.5.1.41 Tween 80 about 30% activation at 1% Aspergillus flavus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 2-mercaptoethanol strong inhibition Aspergillus flavus
3.5.1.41 Ag+
-
 Aspergillus flavus
3.5.1.41 Co2+
-
 Aspergillus flavus
3.5.1.41 Cu2+
-
 Aspergillus flavus
3.5.1.41 DTT strong inhibition Aspergillus flavus
3.5.1.41 EDTA
-
 Aspergillus flavus
3.5.1.41 Fe2+
-
 Aspergillus flavus
3.5.1.41 Fe3+
-
 Aspergillus flavus
3.5.1.41 Hg2+
-
 Aspergillus flavus
3.5.1.41 Mg2+
-
 Aspergillus flavus
3.5.1.41 additional information no product inhibition by acetate up to 70 mM concentration Aspergillus flavus
3.5.1.41 SDS strong inhibition Aspergillus flavus
3.5.1.41 Urea moderate inhibition Aspergillus flavus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.41 additional information
-
 additional information Km and kcat values of the enzyme are determined to be 9.45 mg/ml using colloidal chitin as substrate, Michaelis-Menten kinetics Aspergillus flavus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Aspergillus flavus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 K+ slight activation at 10 mM Aspergillus flavus
3.5.1.41 Mn2+ slight activation Aspergillus flavus
3.5.1.41 additional information no or poor effects by 10-20 mm Na+, Sn2+, and Ni2+ Aspergillus flavus
3.5.1.41 Na+ slight activation at 10 mM Aspergillus flavus
3.5.1.41 Zn2+ slight activation Aspergillus flavus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Aspergillus flavus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.41 native enzyme 3.94fold by ammonium sulfate precipitation, ion exchange chromatography, and gel filtration Aspergillus flavus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.41 additional information the CDA production in SSF increases 1.79fold in comparison to the unoptimized basal level medium. Optimization of significant parameters affecting CDA production in SSF by response surface methodology (RSM) Aspergillus flavus
-
3.5.1.41 mycelium solid-substrate fermentation, wheat bran is used as the substrate Aspergillus flavus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.41 55.91
-
 purified native enzyme, substrate colloidal chitin, pH 6.5, 50°C Aspergillus flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 colloidal chitin + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 glycol chitin + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 N,N',N''-triacetylchitotriose + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 N,N'-diacetylchitobiose + H2O
-
 Aspergillus flavus ?
-
 ?
3.5.1.41 N-acetylglucosamine + H2O
-
 Aspergillus flavus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.41 ? x * 28000, SDS-PAGE Aspergillus flavus

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 CDA
-
 Aspergillus flavus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 50
-
-
 Aspergillus flavus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 40
-
 purified native enzyme, 3 h, stable Aspergillus flavus
3.5.1.41 50
-
 purified native enzyme, 3 h, 40% activity remaining, half-life is 135 min. At temperatures above 50°C, the enzyme stability is significantly reduced Aspergillus flavus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.41 additional information
-
 additional information Km and kcat values of the enzyme are determined to be 26.72/s using colloidal chitin as substrate Aspergillus flavus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 8
-
-
 Aspergillus flavus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.41 2.5 10.5 activity range, profile overview Aspergillus flavus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.41 2.5 10.5 stability range, profile overview. Most stable at pH 7.0-9.0 Aspergillus flavus