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Literature summary extracted from

  • Fukuda, K.; Kiyokawa, Y.; Yanagiuchi, T.; Wakai, Y.; Kitamoto, K.; Inoue, Y.; Kimura, A.
    Purification and characterization of isoamyl acetate-hydrolyzing esterase encoded by the IAH1 gene of Saccharomyces cerevisiae from a recombinant Escherichia coli (2000), Appl. Microbiol. Biotechnol., 53, 596-600 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.112 2-mercaptoethanol 1 mM, 143% of initial activity Saccharomyces cerevisiae
3.1.1.112 dithiothreitol 1 mM, 160% of initial activity Saccharomyces cerevisiae
3.1.1.112 EDTA 1 mM, 133% of initial activity Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.112 expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.112 4-chloromercuribenzoate 0.1 mM, 40% residual activity Saccharomyces cerevisiae
3.1.1.112 diisopropyl fluorophosphate 0.1 mM, no residual activity Saccharomyces cerevisiae
3.1.1.112 Hg2+ 1 mM, no residual activity Saccharomyces cerevisiae
3.1.1.112 iodoacetamide 1 mM, no residual activity Saccharomyces cerevisiae
3.1.1.112 Triton X-100 1%, 72% residual activtiy Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.112 15.3
-
 2-methylpropyl acetate pH 7.0, 25°C Saccharomyces cerevisiae
3.1.1.112 40.3
-
 3-methylbutyl acetate pH 7.0, 25°C Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.112 Saccharomyces cerevisiae P41734
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.112 recombinant protein Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.112 2-methylpropyl acetate + H2O
-
 Saccharomyces cerevisiae 2-methylpropanol + acetate
-
 ?
3.1.1.112 3-methylbutyl acetate + H2O about 80% of the activity with 2-methylpropyl acetate Saccharomyces cerevisiae 3-methylbutanol + acetate
-
 ?
3.1.1.112 isopropyl acetate + H2O about 70% of the activity with 2-methylpropyl acetate Saccharomyces cerevisiae isopropanol + acetate
-
 ?
3.1.1.112 N-propyl acetate + H2O about 85% of the activity with 2-methylpropyl acetate Saccharomyces cerevisiae n-propanol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.112 ? x * 28000, SDS-PAGE, x * 37305, calculated from sequence Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
3.1.1.112 IAH1
-
 Saccharomyces cerevisiae