EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.55 | DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme without the N-terminal signal peptide in Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
3.2.1.145 | DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
3.2.1.213 | expression of C-terminal deletion mutant of Bl1,6Gal with a C-terminal His-tag (Bl1,6Gal-CDELTA248) | Bifidobacterium longum subsp. Longum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.55 | EDTA | inhibits 47% at 1 mM | Bifidobacterium longum subsp. Longum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | additional information | - |
additional information | Km for larch type II arabinogalactan is 7.86 mg/ml for the recombinant enzyme | Bifidobacterium longum subsp. Longum | |
3.2.1.213 | 0.549 | - |
beta-D-Gal(1->6)-beta-D-Gal-(1->6)-D-Gal | pH 4.5, 40°C | Bifidobacterium longum subsp. Longum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.55 | Ca2+ | activates 24% at 1 mM | Bifidobacterium longum subsp. Longum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.213 | 133000 | - |
C-terminal deletion mutant of Bl1,6Gal with a C-terminal His-tag (Bl1,6Gal-CDELTA248), SDS-PAGE | Bifidobacterium longum subsp. Longum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.213 | (1->6)-beta-D-galactooligosaccharide + H2O | Bifidobacterium longum subsp. Longum | degradative enzyme for type II arabinogalactan side chains in cooperation with exo-beta-1,3-galactanase. The enzyme, characterized from the bacterium Bifidobacterium longum, specifically hydrolyses (1->6)-beta-galactobiose from the non-reducing terminal of (1->6)-beta-D-galactooligosaccharides with a degree of polymerization of 3 or higher, using an exo mode of action. The enzyme cannot hydrolyse alpha-L-arabinofuranosylated (1->6)-beta-galactans (as found in arabinogalactans) and does not act on (1->3)-beta-D- or (1->4)-beta-D-galactans | (1->6)-beta-D-galactobiose + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.55 | Bifidobacterium longum subsp. Longum | A0A401ETL2 | - |
- |
3.2.1.145 | Bifidobacterium longum subsp. Longum | A0A401ETL2 | - |
- |
3.2.1.213 | Bifidobacterium longum subsp. Longum | A0A401ETL2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.55 | recombinant C-terminally His-tagged enzyme without the N-terminal signal peptide from Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
3.2.1.145 | recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
3.2.1.213 | C-terminal deletion mutant of Bl1,6Gal with a C-terminal His-tag (Bl1,6Gal-CDELTA248) | Bifidobacterium longum subsp. Longum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.55 | 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Bifidobacterium longum subsp. Longum | 4-nitrophenol + L-arabinofuranose | - |
? | |
3.2.1.55 | arabinan + H2O | release of L-arabinofuranose | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? | |
3.2.1.55 | beta-D-Galp-(1->6)-[alpha-L-Araf-(1->3)]-beta-D-Galp-(1->6)-beta-D-Galp + H2O | recombinant BlArafA releases L-arabinose from the substrate, which is a beta-1,6-galactan side chain containing alpha-1,3-linked L-Araf | Bifidobacterium longum subsp. Longum | L-arabinofuranose + beta-D-Gal-(1->6)-beta-D-Gal-(1->6)-beta-D-Gal | - |
? | |
3.2.1.55 | gum arabic + H2O | release of L-arabinofuranose, low activity | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? | |
3.2.1.55 | larch type II arabinogalactan + H2O | release of L-arabinofuranose, degradation profiles of type II AG | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? | |
3.2.1.55 | methyl alpha-L-Araf-(1->2)-alpha-L-Araf + H2O | low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf | Bifidobacterium longum subsp. Longum | L-arabinofuranose + L-Araf-alpha-O-methyl ether | - |
? | |
3.2.1.55 | methyl alpha-L-Araf-(1->3)-alpha-L-Araf + H2O | - |
Bifidobacterium longum subsp. Longum | L-arabinofuranose + L-Araf-alpha-O-methyl ether | - |
? | |
3.2.1.55 | methyl alpha-L-Araf-(1->5)-alpha-L-Araf + H2O | low activity compared to methyl alpha-L-Araf-(1->3)-alpha-L-Araf | Bifidobacterium longum subsp. Longum | L-arabinofuranose + L-Araf-alpha-O-methyl ether | - |
? | |
3.2.1.55 | additional information | the recombinant alpha-L-arabinofuranosidase releases arabinofuranose (Araf) from alpha-1,3-Araf-substituted beta-1,6-galactooligosaccharides. beta-1,6-Gal2 is additively released from larch type II arabinogalactan (AG) by the combined use of type II AG degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145). Degradative mechanism, overview. The enzyme shows 100, 86.1, and 69.1% specificities for radish root AGP, larch AG, and arabinan, respectively. Arabinoxylan is a poor substrate. BlArafA is an alpha-L-arabinofuranosidase, which recognizes oligosaccharides containing alpha-1,3-linked L-Araf structures in type II AG and arabinan | Bifidobacterium longum subsp. Longum | ? | - |
? | |
3.2.1.55 | radish root arabinogalactan-protein + H2O | release of L-arabinofuranose | Bifidobacterium longum subsp. Longum | L-arabinofuranose + ? | - |
? | |
3.2.1.145 | beta-1,6-Gal2-galactitol + H2O | the enzyme specifically releases beta-1,6-Gal2 but not galactose from beta-1,6-Gal2-Gat | Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + galactitol | - |
? | |
3.2.1.145 | beta-1,6-Gal3 + H2O | - |
Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + D-galactose | - |
? | |
3.2.1.145 | beta-1,6-galactooligosaccharides + H2O | the enzyme releases beta-1,6-Gal2 from beta-1,6-galactooligosaccharides (beta-1,6-Gal>9) in a time-dependent manner with low rates of beta-1,6-Gal3 and beta-1,6-Gal4 | Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + ? | - |
? | |
3.2.1.145 | larch type II arabinogalactan + H2O | degradation profiles of type II AG | Bifidobacterium longum subsp. Longum | ? | - |
? | |
3.2.1.145 | larch type II arabinogalactan + H2O | release of galactobiose, beta-1,6-Gal2, degradation profiles of type II AG | Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + ? | - |
? | |
3.2.1.145 | additional information | the recombinant exo-beta-1,6-galactobiohydrolase specifically releases beta-1,6-galactobiose (beta-1,6-Gal2) from the nonreducing terminal of beta-1,6-galactooligosaccharides. beta-1,6-Gal2 is additively released from larch type II arabinogalactan (AG) by the combined use of type II AG degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145). Degradative mechanism, overview | Bifidobacterium longum subsp. Longum | ? | - |
? | |
3.2.1.213 | (1->6)-beta-D-galactooligosaccharide + H2O | degradative enzyme for type II arabinogalactan side chains in cooperation with exo-beta-1,3-galactanase. The enzyme, characterized from the bacterium Bifidobacterium longum, specifically hydrolyses (1->6)-beta-galactobiose from the non-reducing terminal of (1->6)-beta-D-galactooligosaccharides with a degree of polymerization of 3 or higher, using an exo mode of action. The enzyme cannot hydrolyse alpha-L-arabinofuranosylated (1->6)-beta-galactans (as found in arabinogalactans) and does not act on (1->3)-beta-D- or (1->4)-beta-D-galactans | Bifidobacterium longum subsp. Longum | (1->6)-beta-D-galactobiose + ? | - |
? | |
3.2.1.213 | (1->6)-beta-D-galactooligosaccharide + H2O | the enzyme, characterized from the bacterium Bifidobacterium longum, specifically hydrolyses (1->6)-beta-galactobiose from the non-reducing terminal of (1->6)-beta-D-galactooligosaccharides with a degree of polymerization of 3 or higher, using an exo mode of action. The enzyme cannot hydrolyse alpha-L-arabinofuranosylated (1->6)-beta-galactans (as found in arabinogalactans) and does not act on (1->3)-beta-D- or (1->4)-beta-D-galactans | Bifidobacterium longum subsp. Longum | (1->6)-beta-D-galactobiose + ? | - |
? | |
3.2.1.213 | beta-D-Gal(1->6)-beta-D-Gal-(1->6)-D-Gal + H2O | - |
Bifidobacterium longum subsp. Longum | (1->6)-beta-D-galactobiose + beta-D-galactose | - |
? | |
3.2.1.213 | dearabinosylated larch arabinogalactan + H2O | - |
Bifidobacterium longum subsp. Longum | (1->6)-beta-D-galactobiose + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.55 | ? | x * 133000, recombinant His-tagged enzyme without signal peptide, SDS-PAGE, x * 132981, His-tagged enzyme without signal peptide, sequence calculation | Bifidobacterium longum subsp. Longum |
3.2.1.145 | ? | x * 116000, recombinant His-tagged enzyme, SDS-PAGE, x * 113302, His-tagged enzyme, sequence calculation | Bifidobacterium longum subsp. Longum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.55 | BlAraf | - |
Bifidobacterium longum subsp. Longum |
3.2.1.55 | blArafA | - |
Bifidobacterium longum subsp. Longum |
3.2.1.55 | GH43_22 alpha-L-arabinofuranosidase | - |
Bifidobacterium longum subsp. Longum |
3.2.1.145 | Bl1,3Gal | - |
Bifidobacterium longum subsp. Longum |
3.2.1.145 | Bl1,6Gal | - |
Bifidobacterium longum subsp. Longum |
3.2.1.145 | exo-beta-1,3-galactanase | - |
Bifidobacterium longum subsp. Longum |
3.2.1.145 | exo-beta-1,6-galactobiohydrolase | - |
Bifidobacterium longum subsp. Longum |
3.2.1.145 | GH30_5 exo-beta-1,6-galactobiohydrolase | - |
Bifidobacterium longum subsp. Longum |
3.2.1.145 | GH43_24 exo-beta-1,3-galactanase | - |
Bifidobacterium longum subsp. Longum |
3.2.1.213 | 1,6Gal | - |
Bifidobacterium longum subsp. Longum |
3.2.1.213 | Bl1,6Gal | - |
Bifidobacterium longum subsp. Longum |
3.2.1.213 | exo-beta-1,6-galactobiohydrolase | - |
Bifidobacterium longum subsp. Longum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 50 | - |
recombinant enzyme | Bifidobacterium longum subsp. Longum |
3.2.1.145 | 40 | - |
assay at | Bifidobacterium longum subsp. Longum |
3.2.1.213 | 40 | - |
substrate: beta-D-Gal(1->6)-beta-D-Gal-(1->6)-D-Gal | Bifidobacterium longum subsp. Longum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.55 | 220 | - |
larch type II arabinogalactan | pH 5.5, 40°C, recombinant enzyme | Bifidobacterium longum subsp. Longum | |
3.2.1.213 | 261 | - |
beta-D-Gal(1->6)-beta-D-Gal-(1->6)-D-Gal | pH 4.5, 40°C | Bifidobacterium longum subsp. Longum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.55 | 5.5 | - |
recombinant enzyme | Bifidobacterium longum subsp. Longum |
3.2.1.145 | 4.5 | - |
assay at | Bifidobacterium longum subsp. Longum |
3.2.1.213 | 4.5 | - |
substrate: beta-D-Gal(1->6)-beta-D-Gal-(1->6)-D-Gal | Bifidobacterium longum subsp. Longum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.55 | evolution | the enzyme belongs to the glycosyl hydrolase family 43, GH43, subfamily 5. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) forms a gene cluster with GH30_5 and GH43_22 enzyme candidates, which are conserved in all Bifidobacterium longum strains | Bifidobacterium longum subsp. Longum |
3.2.1.55 | metabolism | GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) | Bifidobacterium longum subsp. Longum |
3.2.1.55 | physiological function | type II arabinogalactan (AG) degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are important for the acquisition of type II AG in Bifidobacterium longum | Bifidobacterium longum subsp. Longum |
3.2.1.145 | evolution | the enzyme belongs to the glycosyl hydrolase family 30, GH30, subfamily 22. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) forms a gene cluster with GH30_5 and GH43_22 enzyme candidates, which are conserved in all Bifidobacterium longum strains | Bifidobacterium longum subsp. Longum |
3.2.1.145 | evolution | the enzyme belongs to the glycosyl hydrolase family 43, GH43, subfamily 24. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal) forms a gene cluster with GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal) and GH43_22 alpha-L-arabinofuranosidase (BlArafA) enzyme candidates, which are conserved in all Bifidobacterium longum strains | Bifidobacterium longum subsp. Longum |
3.2.1.145 | metabolism | GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) | Bifidobacterium longum subsp. Longum |
3.2.1.145 | metabolism | GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55), which are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) | Bifidobacterium longum subsp. Longum |
3.2.1.145 | physiological function | type II arabinogalactan (AG) degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are important for the acquisition of type II AG in Bifidobacterium longum | Bifidobacterium longum subsp. Longum |
3.2.1.213 | physiological function | degradative enzyme for type II arabinogalactan side chains in cooperation with exo-beta-1,3-galactanase | Bifidobacterium longum subsp. Longum |