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Literature summary extracted from

  • Hasona, A.; York, S.; Yomano, L.; Ingram, L.; Shanmugam, K.
    Decreasing the level of ethyl acetate in ethanolic fermentation broths of Escherichia coli KO11 by expression of Pseudomonas putida estZ esterase (2002), Appl. Environ. Microbiol., 68, 2651-2659 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.113 analysis method to rapidly screen bacterial colonies for the presence of esterases which hydrolyze ethyl acetate based on pH change Pseudomonas putida
3.1.1.113 synthesis expression of EstZ in Escherichia coli KO11 reduces the concentration of ethyl acetate in fermentation broth (4.8xa0% ethanol) to less than 20 mg/l Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.113 expression in Escherichia coli Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.113 diethyldicarbonate almost complete inhibition Pseudomonas putida
3.1.1.113 phenylmethylsulfonyl fluoride almost complete inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.113 0.018
-
 Alpha-naphthyl acetate 37°C, pH 7.5 Pseudomonas putida
3.1.1.113 0.054
-
 4-nitrophenyl acetate 37°C, pH 7.5 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.113 36000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.113 Pseudomonas putida Q8KQK1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.113 11
-
 substrate 4-nitrophenylacetate, pH 7.5, 37°C Pseudomonas putida
3.1.1.113 15
-
 substrate alpha-naphthyl caproate, pH 7.5, 37°C Pseudomonas putida
3.1.1.113 33
-
 substrate alpha-naphthyl propionate, pH 7.5, 37°C Pseudomonas putida
3.1.1.113 42
-
 substrate alpha-naphthyl acetate, pH 7.5, 37°C Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.113 4-nitrophenyl acetate + H2O
-
 Pseudomonas putida 4-nitrophenol + acetate
-
 ?
3.1.1.113 alpha-naphthyl acetate + H2O
-
 Pseudomonas putida alpha-naphthol + acetate
-
 ?
3.1.1.113 alpha-naphthyl caproate + H2O
-
 Pseudomonas putida alpha-naphthol + caproate
-
 ?
3.1.1.113 alpha-naphthyl propionate + H2O
-
 Pseudomonas putida alpha-naphthol + propionate
-
 ?
3.1.1.113 ethyl acetate + H2O
-
 Pseudomonas putida ethanol + acetate
-
 ?
3.1.1.113 propyl acetate + H2O
-
 Pseudomonas putida propanol + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.113 monomer 1 * 36000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.1.1.113 estZ
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.113 40
-
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.113 29
-
 Alpha-naphthyl acetate 37°C, pH 7.5 Pseudomonas putida
3.1.1.113 64
-
 4-nitrophenyl acetate 37°C, pH 7.5 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.113 7.5
-
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
3.1.1.113 physiological function enzyme belongs to family IV of lipolytic enzymes and contains the conserved catalytic triad of serine, aspartic acid, and histidine Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.113 1200
-
 4-nitrophenyl acetate 37°C, pH 7.5 Pseudomonas putida
3.1.1.113 1600
-
 Alpha-naphthyl acetate 37°C, pH 7.5 Pseudomonas putida