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Literature summary extracted from

  • Meshram, V.; Saxena, S.; Paul, K.; Gupta, M.; Kapoor, N.
    Production, purification and characterisation of a potential fibrinolytic protease from endophytic Xylaria curta by solid substrate fermentation (2017), Appl. Biochem. Biotechnol., 181, 1496-1512 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.72 2-mercaptoethanol about 65% residual activity at 2 mM Xylaria curta
3.4.24.72 Al3+ about 80% residual activity at 2 mM Xylaria curta
3.4.24.72 Co2+ about 80% residual activity at 2 mM Xylaria curta
3.4.24.72 Cu2+ about 55% residual activity at 2 mM Xylaria curta
3.4.24.72 EDTA complete inhibition at 2 mM Xylaria curta
3.4.24.72 EGTA complete inhibition at 2 mM Xylaria curta
3.4.24.72 Fe2+ complete inhibition at 2 mM Xylaria curta
3.4.24.72 K+ about 80% residual activity at 2 mM Xylaria curta
3.4.24.72 leupeptin about 75% residual activity at 2 mM Xylaria curta
3.4.24.72 Mg2+ about 75% residual activity at 2 mM Xylaria curta
3.4.24.72 Mn2+ about 70% residual activity at 2 mM Xylaria curta
3.4.24.72 Na+ about 85% residual activity at 2 mM Xylaria curta
3.4.24.72 phenylmethylsulfonyl fluoride about 70% residual activity at 2 mM Xylaria curta
3.4.24.72 tosyl-phenylalanine chloromethyl ketone about 80% residual activity at 2 mM Xylaria curta
3.4.24.72 Zn2+ complete inhibition at 2 mM Xylaria curta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.72 0.3266
-
 azocasein at pH 7.8 and 37°C Xylaria curta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.72 Ca2+ about 105% activity at 2 mM Xylaria curta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.72 fibrinogen + H2O Xylaria curta the enzyme exhibits cleavage of Aalphaand Bbeta chains of fibrin(ogen) and has no effect on gamma chain fibrin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.72 Xylaria curta
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.72 ammonium sulfate precipitation and Sephacryl S-300 gel filtration Xylaria curta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.72 additional information
-
 specific activity of 9.22 units/mg after 8.37fold purification. One unit is defined as the amount of the enzyme producing acid-soluble material from azocasein to cause an increase in absorbance of 0.001 at 660 nm per min Xylaria curta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.72 azocasein + H2O
-
 Xylaria curta ?
-
 ?
3.4.24.72 bovine fibrinogen + H2O
-
 Xylaria curta fibrin + ?
-
 ?
3.4.24.72 fibrinogen + H2O the enzyme exhibits cleavage of Aalphaand Bbeta chains of fibrin(ogen) and has no effect on gamma chain Xylaria curta fibrin + ?
-
 ?
3.4.24.72 additional information the enzyme does not display any detectable hydrolysis of gamma chain of fibrin over a period of 180 min Xylaria curta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.72 monomer 1 * 33000, SDS-PAGE Xylaria curta

Synonyms

EC Number Synonyms Comment Organism
3.4.24.72 fibrinolytic protease
-
 Xylaria curta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.72 35
-
-
 Xylaria curta

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.24.72 25 55 about 75% activity 25°C, about 80% activity 30°C, 100% activity at 35°C, about 80% activity 40°C, about 60% activity 45°C, about 50% activity 50°C, about 45% activity 55°C. At 60°C, the activity of the enzyme is completely lost Xylaria curta

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.72 60
-
 at 60°C, the activity of the enzyme is completely lost Xylaria curta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.72 8
-
-
 Xylaria curta

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.72 5 9 more than 60% activity between pH 5.0 and 9.0, about 40% activity at pH 10.0, no activity at pH 4.0 Xylaria curta