Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Huang, W.Q.; Zhong, L.F.; Meng, Z.Z.; You, Z.J.; Li, J.Z.; Luo, X.C.
    The structure and enzyme characteristics of a recombinant leucine aminopeptidase rLap1 from Aspergillus sojae and its application in debittering (2015), Appl. Biochem. Biotechnol., 177, 190-206 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.1 food industry application of recombinant leucine aminopeptidase rLap1 from Aspergillus sojae in debittering Aspergillus sojae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 gene lap1, recombinant overexpression of truncated Lap1 without a signal peptide in Pichia pastoris Aspergillus sojae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 EDTA significant inhibition, Co2+, Mn2+, and Ca2+ ions, but not Zn2+ ions, restore the enzyme activity Aspergillus sojae

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Aspergillus sojae Q8J2N2
-
-
3.4.11.1 Aspergillus sojae GIM3.30 Q8J2N2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 Arg 4-nitroanilide + H2O
-
 Aspergillus sojae Arg + 4-nitroaniline
-
 ?
3.4.11.1 Arg 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Arg + 4-nitroaniline
-
 ?
3.4.11.1 Leu 4-nitroanilide + H2O
-
 Aspergillus sojae Leu + 4-nitroaniline
-
 ?
3.4.11.1 Leu 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Leu + 4-nitroaniline
-
 ?
3.4.11.1 Lys 4-nitroanilide + H2O
-
 Aspergillus sojae Lys + 4-nitroaniline
-
 ?
3.4.11.1 Lys 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Lys + 4-nitroaniline
-
 ?
3.4.11.1 Met 4-nitroanilide + H2O
-
 Aspergillus sojae Met + 4-nitroaniline
-
 ?
3.4.11.1 Met 4-nitroanilide + H2O
-
 Aspergillus sojae GIM3.30 Met + 4-nitroaniline
-
 ?
3.4.11.1 additional information rLap1 shows the highest activity against Arg-pNA, followed by Leu-, Lys-, Met-, and Phe-4-nitroanilide in descending order Aspergillus sojae ?
-
 ?
3.4.11.1 additional information rLap1 shows the highest activity against Arg-pNA, followed by Leu-, Lys-, Met-, and Phe-4-nitroanilide in descending order Aspergillus sojae GIM3.30 ?
-
 ?
3.4.11.1 Phe 4-nitroanilide + H2O
-
 Aspergillus sojae Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 36700, recombinant enzyme lacking the signal peptide, SDS-PAGE Aspergillus sojae

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 LAP1
-
 Aspergillus sojae
3.4.11.1 leucine aminopeptidase
-
 Aspergillus sojae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 50
-
 recombinant enzyme Aspergillus sojae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.1 50
-
 purified recombinant enzyme, 1 h, 50% activity remaining Aspergillus sojae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
 recombinant enzyme Aspergillus sojae

General Information

EC Number General Information Comment Organism
3.4.11.1 additional information recombinant Lap1 enzyme has a conserved functional charge/dipole complex and a hydrogen bond network of Zn2-D179-S228-Q177-D229-S158 around its active center. An acidic Asp residue is found at the bottom of the substrate binding pocket, which explains its preference for basic N-terminal amino acid substrates such as Arg and Lys. Three-dimensional structure modelling of rLap1 and substrate-docking analysis Aspergillus sojae
3.4.11.1 physiological function enzyme Lap1 improves the degree of hydrolysis of casein and soy protein hydrolysates and also decreases their bitterness, indicating its potential utility in food production Aspergillus sojae