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Literature summary extracted from

  • Stowell, A.I.; James, D.I.; Waddell, I.D.; Bennett, N.; Truman, C.; Hardern, I.M.; Ogilvie, D.J.
    A high-throughput screening-compatible homogeneous time-resolved fluorescence assay measuring the glycohydrolase activity of human poly(ADP-ribose) glycohydrolase (2016), Anal. Biochem., 503, 58-64 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.143 gene PARG, single gene, recombinant expression of C-terminally His-tagged hPARG in Escherichia coli BL21(DE3) Gold Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.143 additional information development of a high-throughput homogeneous time-resolved fluorescence (HTRF) assay method allows high-throughput screening for the identification and advancement of multiple validated series of tool compounds for PARG inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.143 poly(ADP-ribose) + H2O Homo sapiens
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 ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.143 Homo sapiens Q86W56
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.143 recombinant C-terminally His-tagged hPARG from Escherichia coli BL21(DE3) Gold by nickel affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.143 additional information development of a high-throughput homogeneous time-resolved fluorescence (HTRF) assay method, 6His-TEV-PAR(32:1)PARP-1(2-1014) substrate, combinantion with the activity of poly(ADP-ribose) polymerase, PARP. Enzyme PARG binds to PARylated PARP-1 and cleaves off the PAR moiety, which activates PARP-1 preventing the production of a fluorescent signal. Inactive PARP-1 leads to the production of the measurable fluoresecnt signal, mechanism and evaluation, overview Homo sapiens ?
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 ?
3.2.1.143 poly(ADP-ribose) + H2O
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 Homo sapiens ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.143 PARG
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 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.143 26
-
 assay at Homo sapiens

General Information

EC Number General Information Comment Organism
3.2.1.143 physiological function poly(ADP-ribosyl)ation (PARylation) is a transient posttranslational modification that generates a signaling mechanism with diverse roles within molecular and cellular processes. PAR chains remaining from DNA repair are broken down by the enzyme poly(ADP-ribose) glycohydrolase (PARG). PARG catalyzes the hydrolysis of endo- and exoglycosidic bonds within the poly(ADP-ribose) (PAR) polymers Homo sapiens