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Literature summary extracted from

  • Eustis, I.C.; Huang, J.; Pilkerton, M.E.; Whedon, S.D.; Chatterjee, C.
    A time-resolved Foerster resonance energy transfer assay to measure activity of the deamidase of the prokaryotic ubiquitin-like protein (2015), Anal. Biochem., 487, 27-29 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.5.1.119 medicine since Dop is necessary for persistent infection by Mycobacterium tuberculosis, its selective inhibition holds potential for tuberculosis therapy Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.119
-
 Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.119 additional information in order to facilitate high-throughput screens for Dop inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme Corynebacterium glutamicum
3.5.1.119 additional information in order to facilitate high-throughput screens for Dop inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.119 Corynebacterium glutamicum Q8NQE1
-
-
3.5.1.119 Corynebacterium glutamicum ATCC 13032 Q8NQE1
-
-
3.5.1.119 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.119
-
 Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
3.5.1.119 deamidase of Pup
-
 Mycobacterium tuberculosis
3.5.1.119 deamidase of Pup
-
 Corynebacterium glutamicum
3.5.1.119 Dop
-
 Mycobacterium tuberculosis
3.5.1.119 Dop
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.119 37
-
 assay at Mycobacterium tuberculosis
3.5.1.119 37
-
 assay at Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.119 7.4
-
 assay at Mycobacterium tuberculosis
3.5.1.119 7.4
-
 assay at Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
3.5.1.119 ATP absolutely required for enzyme function Mycobacterium tuberculosis
3.5.1.119 ATP absolutely required for enzyme function Corynebacterium glutamicum

General Information

EC Number General Information Comment Organism
3.5.1.119 metabolism the enzyme is essential for the infectivity of Mycobacterium tuberculosisis Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.119 additional information
-
 additional information in order to facilitate high-throughput screens for Dop inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme Corynebacterium glutamicum
3.5.1.119 additional information
-
 additional information in order to facilitate high-throughput screens for inhibitors, a time-resolved Förster resonance energy transfer-based assay for enzyme (Dop) function is deleveloped. The time-resolved Förster resonance energy transfer-based assay is successfully applied to determine the Michaelis constant for ATP-binding and to test the co-factor tolerance of the enzyme Mycobacterium tuberculosis