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Literature summary extracted from
- (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB) from fatty acid degradation operon of Ralstonia eutropha H16 (2014), AMB Express, 4, 69 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.17 | expression in Escherichia coli | Cupriavidus necator |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.17 | acetyl-CoA | 0.1 mM, 70% inhibition | Cupriavidus necator |  |
| 4.2.1.17 | CoA | 0.1 mM, 30% inhibition | Cupriavidus necator | |
| 4.2.1.17 | propanoyl-CoA | 0.1 mM, 60% inhibition | Cupriavidus necator | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.17 | 87000 | - |
gel filtration | Cupriavidus necator |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.17 | Cupriavidus necator | Q0KEG0 | bifunctional (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase, cf. EC 3.1.1.35 | - |
| 4.2.1.17 | Cupriavidus necator DSM 428 | Q0KEG0 | bifunctional (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase, cf. EC 3.1.1.35 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.17 | 98 | - |
pH 7, 30°C | Cupriavidus necator |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.17 | trans-crotonyl-CoA + H2O | - |
Cupriavidus necator | (S)-3-hydroxybutanoyl-CoA | - |
? | |
| 4.2.1.17 | trans-crotonyl-CoA + H2O | - |
Cupriavidus necator DSM 428 | (S)-3-hydroxybutanoyl-CoA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.17 | monomer | 1 * 8590, calculated from sequence, 1 * 86000, SDS-PAGE | Cupriavidus necator |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.17 | FadB' | - |
Cupriavidus necator |
| 4.2.1.17 | H16_A0461 | - |
Cupriavidus necator |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.1.17 | Cupriavidus necator | calculated from sequence | - |
7.7 |
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Release 2023.1 (January 2023)
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