Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sommer, T.; Bjerregaard-Andersen, K.; Simensen, S.M.; Jensen, J.K.; Jochimsen, B.; Riss, P.J.; Etzerodt, M.; Morth, J.P.
    Enzymatic detection and quantification assay of isatin, a putative stress biomarker in blood (2015), ACS Chem. Neurosci., 6, 1353-1360 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.2.20 analysis a fluorescence-based enzymatic assay is developed that can quantify isatin, a putative stress biomarkerin blood samples. A phase extraction of isatin followed by a second phase extraction combined with an enzymatic reaction performed by an isatin hydrolase is used to extract and quantify isatin in whole blood samples Roseibium aggregatum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.20 recombinantly produced in Escherichia coli Roseibium aggregatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.20 Cu2+ 35% of the activity compared to Mn2+ Roseibium aggregatum
3.5.2.20 Mn2+ the enzyme is highly selective for Mn2+ with a dissociation constant determined to be 0.0095 mM Roseibium aggregatum
3.5.2.20 Ni2+ 30% of the activity compared to Mn2+ Roseibium aggregatum

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.20 Roseibium aggregatum A0NLY7
-
-
3.5.2.20 Roseibium aggregatum ATCC 25650 A0NLY7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.20 isatin + H2O
-
 Roseibium aggregatum isatinate
-
 ?
3.5.2.20 isatin + H2O
-
 Roseibium aggregatum ATCC 25650 isatinate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.20 IH-b
-
 Roseibium aggregatum
3.5.2.20 isatin hydrolase b
-
 Roseibium aggregatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.2.20 60
-
 t1/2: 9 min Roseibium aggregatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.20 8.3
-
-
 Roseibium aggregatum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.20 7 9.5 pH 7.0: about 50% of maximal activity, pH 9.5: about 55% of maximal activity Roseibium aggregatum