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Literature summary extracted from

  • Gama, S.; Vogt, M.; Kalina, T.; Hupp, K.; Hammerschmidt, F.; Pallitsch, K.; Zechel, D.
    An oxidative pathway for microbial utilization of methylphosphonic acid as a phosphate source (2019), ACS Chem. Biol., 14, 735-741 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.89 expression in Escherichia coli Gimesia maris
1.14.11.71 expressed in Escherichia coli Gimesia maris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.89 0.026
-
 (hydroxymethyl)phosphonate pH 7.0, 25°C Gimesia maris
1.13.11.89 0.13
-
 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid pH 7.0, 25°C Gimesia maris
1.13.11.89 0.28
-
 (R)-1-hydroxyethylphosphonic acid pH 7.0, 25°C Gimesia maris
1.14.11.71 0.011
-
 2-oxoglutarate at pH 7.0 and 25°C Gimesia maris
1.14.11.71 1.5
-
 methylphosphonate at pH 7.0 and 25°C Gimesia maris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.89 Iron Km value 0.010 mM for Fe2+ Gimesia maris
1.14.11.71 Fe2+ required for activity, Km value of 0.004 mM Gimesia maris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.89 (hydroxymethyl)phosphonate + O2 Gimesia maris
-
 formate + phosphate
-
 ?
1.13.11.89 (hydroxymethyl)phosphonate + O2 Gimesia maris DSM 8797
-
 formate + phosphate
-
 ?
1.14.11.71 methylphosphonate + 2-oxoglutarate + O2 Gimesia maris
-
 hydroxymethylphosphonate + succinate + CO2
-
 ?
1.14.11.71 methylphosphonate + 2-oxoglutarate + O2 Gimesia maris DSM 8797
-
 hydroxymethylphosphonate + succinate + CO2
-
 ?
1.14.11.71 additional information Gimesia maris 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates ?
-
-
1.14.11.71 additional information Gimesia maris DSM 8797 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.89 Gimesia maris
-
-
-
1.13.11.89 Gimesia maris DSM 8797
-
-
-
1.14.11.71 Gimesia maris
-
-
-
1.14.11.71 Gimesia maris DSM 8797
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.71 Ni-NTA column chromatography Gimesia maris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.89 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid + O2 no substrate: (1S)-[2-amino-1-hydroxyethyl]phosphonic acid Gimesia maris aminoacetate + phosphate
-
 ?
1.13.11.89 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid + O2 no substrate: (1S)-[2-amino-1-hydroxyethyl]phosphonic acid Gimesia maris DSM 8797 aminoacetate + phosphate
-
 ?
1.13.11.89 (hydroxymethyl)phosphonate + O2
-
 Gimesia maris formate + phosphate
-
 ?
1.13.11.89 (hydroxymethyl)phosphonate + O2
-
 Gimesia maris DSM 8797 formate + phosphate
-
 ?
1.13.11.89 (R)-1-hydroxyethylphosphonic acid + O2
-
 Gimesia maris acetate + phosphate
-
 ?
1.13.11.89 (R)-1-hydroxyethylphosphonic acid + O2
-
 Gimesia maris DSM 8797 acetate + phosphate
-
 ?
1.14.11.71 methylphosphonate + 2-oxoglutarate + O2
-
 Gimesia maris hydroxymethylphosphonate + succinate + CO2
-
 ?
1.14.11.71 methylphosphonate + 2-oxoglutarate + O2
-
 Gimesia maris DSM 8797 hydroxymethylphosphonate + succinate + CO2
-
 ?
1.14.11.71 additional information 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates Gimesia maris ?
-
-
1.14.11.71 additional information 2-aminoethylphosphonic acid, ethylphosphonic acid, aminomethylphosphonic acid, phosphonoalanine and glyphosate are not substrates Gimesia maris DSM 8797 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.13.11.89 phnZ1
-
 Gimesia maris
1.14.11.71 phnY*
-
 Gimesia maris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.89 25
-
 assay at Gimesia maris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.89 0.55
-
 (hydroxymethyl)phosphonate pH 7.0, 25°C Gimesia maris
1.13.11.89 1.54
-
 (R)-1-hydroxyethylphosphonic acid pH 7.0, 25°C Gimesia maris
1.13.11.89 2.5
-
 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid pH 7.0, 25°C Gimesia maris
1.14.11.71 0.36
-
 methylphosphonate at pH 7.0 and 25°C Gimesia maris
1.14.11.71 0.42
-
 2-oxoglutarate at pH 7.0 and 25°C Gimesia maris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.89 7
-
 assay at Gimesia maris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.89 18
-
 (R)-1-hydroxyethylphosphonic acid pH 7.0, 25°C Gimesia maris
1.13.11.89 20
-
 (1R)-[2-amino-1-hydroxyethyl]phosphonic acid pH 7.0, 25°C Gimesia maris
1.13.11.89 21
-
 (hydroxymethyl)phosphonate pH 7.0, 25°C Gimesia maris
1.14.11.71 0.24
-
 methylphosphonate at pH 7.0 and 25°C Gimesia maris
1.14.11.71 80
-
 2-oxoglutarate at pH 7.0 and 25°C Gimesia maris