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Literature summary extracted from

  • Dias, F.F.G.; Santos Aguilar, J.G.D.; Sato, H.H.
    L-Asparaginase from Aspergillus spp. production based on kinetics, thermal stability and biochemical characterization (2019), 3 Biotech, 9, 289 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.1 Cu2+ 10 mM Aspergillus niger
3.5.1.1 Cu2+ 10 mM Aspergillus oryzae
3.5.1.1 iodoacetamide
-
 Aspergillus niger
3.5.1.1 iodoacetamide
-
 Aspergillus oryzae
3.5.1.1 additional information not inhibitory: high NaCl conditions Aspergillus niger
3.5.1.1 additional information not inhibitory: high NaCl conditions Aspergillus oryzae
3.5.1.1 p-chloromercuribenzoate
-
 Aspergillus niger
3.5.1.1 p-chloromercuribenzoate
-
 Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.1 1.41
-
 L-asparagine pH 8.0, 40°C Aspergillus niger
3.5.1.1 2.1
-
 L-asparagine pH 8.0, 40°C Aspergillus oryzae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.1 Mn2+ activation Aspergillus niger
3.5.1.1 Mn2+ activation Aspergillus oryzae

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.1 Aspergillus niger
-
-
-
3.5.1.1 Aspergillus niger LBA 02
-
-
-
3.5.1.1 Aspergillus oryzae
-
-
-
3.5.1.1 Aspergillus oryzae CCTZ 3940
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.1 L-asparagine + H2O
-
 Aspergillus niger L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Aspergillus oryzae L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Aspergillus oryzae CCTZ 3940 L-aspartate + NH3
-
 ?
3.5.1.1 L-asparagine + H2O
-
 Aspergillus niger LBA 02 L-aspartate + NH3
-
 ?
3.5.1.1 additional information L-glutamine is a poor substrate Aspergillus niger ?
-
 ?
3.5.1.1 additional information no substrate: L-glutamine Aspergillus oryzae ?
-
 ?
3.5.1.1 additional information no substrate: L-glutamine Aspergillus oryzae CCTZ 3940 ?
-
 ?
3.5.1.1 additional information L-glutamine is a poor substrate Aspergillus niger LBA 02 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.1 40 50
-
 Aspergillus niger
3.5.1.1 40 50
-
 Aspergillus oryzae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.1 50
-
 stable for 60 min Aspergillus niger
3.5.1.1 50
-
 stable for 60 min Aspergillus oryzae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.1 5 8
-
 Aspergillus niger
3.5.1.1 5 8
-
 Aspergillus oryzae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.1 5 8
-
 Aspergillus niger
3.5.1.1 5 8
-
 Aspergillus oryzae

General Information

EC Number General Information Comment Organism
3.5.1.1 physiological function comparison of native L-asparaginases from Aspergillus strains Aspergillus niger
3.5.1.1 physiological function comparison of native L-asparaginases from Aspergillus strains Aspergillus oryzae