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Literature summary extracted from
- Cloning, expression and characterization of L-asparaginase from Pseudomonas fluorescens for large scale production in E. coli BL21 (2015), 3 Biotech, 5, 975-981 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.1 | expression in Escherichia coli | Pseudomonas fluorescens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.1.1 | 110 | - |
L-asparagine | pH 6.3, 28°C | Pseudomonas fluorescens |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.1 | Pseudomonas fluorescens | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.1 | L-asparagine + H2O | - |
Pseudomonas fluorescens | L-aspartate + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.1 | ? | x * 21000 + x * 35000 + x * 40000 + x * 45000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 34 | - |
- |
Pseudomonas fluorescens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 28 | - |
half-life 35 h | Pseudomonas fluorescens |
| 3.5.1.1 | 37 | - |
half-life 45.5 h | Pseudomonas fluorescens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.1 | 6.3 | - |
- |
Pseudomonas fluorescens |
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