Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pokorna, J.; Pachl, P.; Karlukova, E.; Hejdanek, J.; Rezacova, P.; Machara, A.; Hudlicky, J.; Konvalinka, J.; Kozisek, M.
    Kinetic, Thermodynamic, and structural analysis of drug resistance mutations in neuraminidase from the 2009 pandemic Influenza virus (2018), Viruses, 10, E339 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expressed in Drosophila melanogaster Schneider S2 cells influenza A virus H1N1

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.18 mutant enzymes in complex with oseltamivir carboxylate, hanging drop vapor diffusion method, mutant I223V: 0.1 M HEPES pH 6.75, 7% (w/v) PEG 8000, mutant S247N: 0.1 M HEPES pH 6.7, 8.5% (w/v) PEG 8000, mutant H275Y: 0.1 M HEPES pH 7.0, 8% (w/v) PEG 8000, mutant I223V/H275Y: 0.1 M HEPES pH 7.0, 7% (w/v) PEG 8000, and mutant S247N/H275Y: 0.1 M HEPES pH 7.5, 5% (w/v) PEG 8000 influenza A virus H1N1

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.18 H275Y the major oseltamivir resistance mutation H275Y causes a significant decrease in the enzyme's ability to bind oseltamivir influenza A virus H1N1
3.2.1.18 H275Y/I223V the mutations result in extreme impairment of oseltamivir's inhibition potency influenza A virus H1N1
3.2.1.18 H275Y/S247N the mutations result in extreme impairment of oseltamivir's inhibition potency influenza A virus H1N1
3.2.1.18 I223V the substitution alone confers only a moderate reduction in oseltamivir affinity influenza A virus H1N1
3.2.1.18 S247N the substitution alone confers only a moderate reduction in oseltamivir affinity influenza A virus H1N1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.18 oseltamivir
-
 influenza A virus H1N1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.18 1.1
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid wild type enzyme, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 1.1
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 1.1
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme S247N, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 1.2
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 1.3
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 3.3
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/S247N, at 37°C, pH not specified in the publication influenza A virus H1N1

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 influenza A virus H1N1 C3W6G3 A/California/07/2009
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 Strep-Tactin resin column chromatography influenza A virus H1N1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid + H2O
-
 influenza A virus H1N1 4-methylumbelliferone + alpha-D-N-acetylneuraminic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 neuraminidase
-
 influenza A virus H1N1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.18 0.045
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.054
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.1
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/S247N, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.17
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme S247N, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.6
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.9
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid wild type enzyme, at 37°C, pH not specified in the publication influenza A virus H1N1

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.18 0.000024
-
 oseltamivir wild type enzyme, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.00045
-
 oseltamivir mutant enzyme S247N, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.0007
-
 oseltamivir mutant enzyme I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.027
-
 oseltamivir mutant enzyme H275Y, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.094
-
 oseltamivir mutant enzyme H275Y/I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.22
-
 oseltamivir mutant enzyme H275Y/S247N, at 37°C, pH not specified in the publication influenza A virus H1N1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.18 0.031
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/S247N, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.035
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y/I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.048
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme H275Y, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.16
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme S247N, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.51
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid mutant enzyme I223V, at 37°C, pH not specified in the publication influenza A virus H1N1
3.2.1.18 0.82
-
 4-methylumbelliferyl alpha-D-N-acetylneuraminic acid wild type enzyme, at 37°C, pH not specified in the publication influenza A virus H1N1