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Literature summary extracted from

  • Trummal, K.; Samel, M.; Aaspollu, A.; Tonismaegi, K.; Titma, T.; Subbi, J.; Siigur, J.; Siigur, E.
    5'-Nucleotidase from Vipera lebetina venom (2015), Toxicon, 93, 155-163 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.5 Ca2+ 89.5% residual activity at 5 mM Macrovipera lebetina
3.1.3.5 Cu2+ 3.9% residual activity at 5 mM Macrovipera lebetina
3.1.3.5 EDTA
-
 Macrovipera lebetina
3.1.3.5 Fe3+ 3.3% residual activity at 5 mM Macrovipera lebetina
3.1.3.5 Mg2+ 80.9% residual activity at 5 mM Macrovipera lebetina
3.1.3.5 vanillic acid
-
 Macrovipera lebetina
3.1.3.5 Zn2+ 2.1% residual activity at 5 mM Macrovipera lebetina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.5 Mn2+ the enzyme activity is enhanced by Mn2+ (170% activity at 5 mM) Macrovipera lebetina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.5 120000
-
 gel filtration Macrovipera lebetina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.5 5'-AMP + H2O Macrovipera lebetina
-
 adenosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.5 Macrovipera lebetina W8EFS0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.5 DEAE-Toyopearl column chromatography, CM52 cellulose column chromatography, Superdex 200 gel filtration, and Sephadex G-100 gel filtration Macrovipera lebetina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.5 venom
-
 Macrovipera lebetina
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.5 5'-AMP + H2O
-
 Macrovipera lebetina adenosine + phosphate
-
 ?
3.1.3.5 5'-AMP + H2O best substrate Macrovipera lebetina adenosine + phosphate
-
 ?
3.1.3.5 5'-GMP + H2O
-
 Macrovipera lebetina guanosine + phosphate
-
 ?
3.1.3.5 ADP + H2O
-
 Macrovipera lebetina 5'-AMP + phosphate
-
 ?
3.1.3.5 ATP + H2O lowest activity Macrovipera lebetina ADP + phosphate
-
 ?
3.1.3.5 additional information no activity with 3'-AMP, 3',5'-cAMP, and 3',5'-cGMP Macrovipera lebetina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.5 homodimer 2 * 60000, SDS-PAGE Macrovipera lebetina

Synonyms

EC Number Synonyms Comment Organism
3.1.3.5 5'-NT
-
 Macrovipera lebetina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.5 10 60 the enzyme is stable from 10-37°C. The activity of the enzyme decreases after heating at 37°C, at 50°C approximately two third of the activity is left, at 60°C the activity is almost entirely lost Macrovipera lebetina

General Information

EC Number General Information Comment Organism
3.1.3.5 physiological function the enzyme strongly inhibits ADP- or collagen-induced platelet aggregation Macrovipera lebetina