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Literature summary extracted from

  • Dong, Z.; Zhang, L.; Qiao, M.; Ge, J.; Liu, A.; Li, Z.
    A label-free assay for T4 polynucleotide kinase/phosphatase activity and its inhibitors based on poly(thymine)-templated copper nanoparticles (2015), Talanta, 146, 253-258 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.78 analysis label-free fluorescent assay for T4 polynucleotidekinase/phosphatase PNKP activity using poly(thymine)-templated fluorescent copper nanoparticles as a fluorescent indicator. A T-rich hairpin primer with a 3'-phosphoryl end, serves as both the substrate for T4 PNKP and DNA template for the formation of fluorescent copper nanoparticles. Upon hydrolysis by T4 PNKP, the resulting hairpin primer with a 3'-hydroxyl end is elongated to form a long double-strand product by DNA polymerase, which prohibits the formation of fluorescent copper nanoparticles Tequatrovirus T4
3.1.3.32 analysis label-free fluorescent assay for T4 polynucleotide kinase/phosphatase PNKP activity using poly(thymine)-templated fluorescent copper nanoparticles as a fluorescent indicator. A T-rich hairpin primer with a 3'-phosphoryl end, serves as both the substrate for T4 PNKP and DNA template for the formation of fluorescent copper nanoparticles. Upon hydrolysis by T4 PNKP, the resulting hairpin primer with a 3'-hydroxyl end is elongated to form a long double-strand product by DNA polymerase, which prohibits the formation of fluorescent copper nanoparticles Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Tequatrovirus T4 P06855
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3.1.3.32 Tequatrovirus T4 P06855 cf. 2.7.1.78
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