EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.373 | synthesis | use of enzyme to generate a platform for flavonoid glucosylation. Engineered variants show remarkable ability for luteolin, morin and naringenin glucosylation with conversion ranging from 30% to 90% | Leuconostoc mesenteroides |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.4.1.B77 | additional information | engineering of truncated variant DELTAN123-GBD-CD2 for flavonoid glucoside diversification. Mutantions at residues W2135, F2136, F2163 and L2166 lead to improved promiscuity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin | Leuconostoc citreum |
2.4.1.373 | W2135F | mutant displays 3-4fold improved activity with naringenin | Leuconostoc mesenteroides |
2.4.1.373 | W2135G | mutant displays 3-4fold improved activity with naringenin | Leuconostoc mesenteroides |
2.4.1.373 | W2135G | mutant synthesizes four times more morin glucoside than the wild-type | Leuconostoc mesenteroides |
2.4.1.373 | W2135I/F2136Y | mutant displays 3-4fold improved activity with naringenin, about 60% conversion. Mutant converts nearly 3-times more naringenin than luteolin (59% and 17%, conversion, respectively) | Leuconostoc mesenteroides |
2.4.1.373 | W2135L-F2136L | mutant significantly synthesizes quercetin di-glucoside | Leuconostoc mesenteroides |
2.4.1.373 | W2135V | mutant displays 3-4fold improved activity with naringenin | Leuconostoc mesenteroides |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.B77 | Leuconostoc citreum | - |
- |
- |
2.4.1.B77 | Leuconostoc citreum NRRL B-1299 | - |
- |
- |
2.4.1.373 | Leuconostoc mesenteroides | Q8G9Q2 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.B77 | additional information | enzyme additionally has transglucosylation activity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin | Leuconostoc citreum | ? | - |
? | |
2.4.1.B77 | additional information | enzyme additionally has transglucosylation activity towards flavonoids including quercetin, luteolin, apigenin, morin, chrysin and naringenin | Leuconostoc citreum NRRL B-1299 | ? | - |
? | |
2.4.1.373 | sucrose + luteolin | - |
Leuconostoc mesenteroides | D-fructose + luteolin glucoside | - |
? | |
2.4.1.373 | sucrose + morin | - |
Leuconostoc mesenteroides | D-fructose + morin glucoside | one mono-glucoside, three di-glucosides and one tri-glucoside | ? | |
2.4.1.373 | sucrose + naringenin | - |
Leuconostoc mesenteroides | D-fructose + naringenin glucoside | one naringenin mono-glucoside, two di-glucosides and one tri-glucoside | ? | |
2.4.1.373 | sucrose + quercetin | - |
Leuconostoc mesenteroides | D-fructose + quercetin glucoside | - |
? |