EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.81 | Methylcyclohexane | about 250% activity at 100% (v/v) | Saccharolobus solfataricus | |
3.1.8.1 | Methylcyclohexane | about 250% activity at 100% (v/v) | Saccharolobus solfataricus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.81 | expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
3.1.8.1 | expressed in Escherichia coli BL21(DE3) cells | Saccharolobus solfataricus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.1.81 | W263I | the mutant has increased lactonase and phosphotriesterase activities compared to the wild type | Saccharolobus solfataricus |
3.1.8.1 | W263I | the mutant has increased lactonase and phosphotriesterase activities compared to the wild type | Saccharolobus solfataricus |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.81 | with the liquid enzyme, the remaining activity decreases down to 40% at the highest dose of beta-radiation (100 kGy) | Saccharolobus solfataricus |
3.1.8.1 | with the liquid enzyme, the remaining activity decreases down to 40% at the highest dose of beta-radiation (100 kGy) | Saccharolobus solfataricus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.81 | petroleum ether | petroleum ether slightly reduces the activity down to 78% | Saccharolobus solfataricus | |
3.1.1.81 | Toluene | toluene slightly reduced the activity down to 74% | Saccharolobus solfataricus | |
3.1.1.81 | xylene | xylene decreases the enzyme activity by more than 75% | Saccharolobus solfataricus | |
3.1.8.1 | petroleum ether | petroleum ether slightly reduces the activity down to 78% | Saccharolobus solfataricus | |
3.1.8.1 | Toluene | toluene slightly reduces the activity down to 74% | Saccharolobus solfataricus | |
3.1.8.1 | xylene | xylene decreases the enzyme activity by more than 75% | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.81 | an N-acyl-L-homoserine lactone + H2O | Saccharolobus solfataricus | - |
an N-acyl-L-homoserine | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
3.1.1.81 | additional information | the enzyme activity is not affected by acetone, acetonitrile, butanone, butyl acetate, chloroform, dichloromethane, diethyl ether, ethanol, ethyl acetate, isopropanol, methanol, and methoxypropanol | Saccharolobus solfataricus |
3.1.8.1 | additional information | the enzyme activity is not affected by acetone, acetonitrile, butanone, butyl acetate, chloroform, dichloromethane, diethyl ether, ethanol, ethyl acetate, isopropanol, methanol, and methoxypropanol | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.81 | Saccharolobus solfataricus | - |
Sulfolobus solfataricus | - |
3.1.8.1 | Saccharolobus solfataricus | - |
Sulfolobus solfataricus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.81 | an N-acyl-L-homoserine lactone + H2O | - |
Saccharolobus solfataricus | an N-acyl-L-homoserine | - |
? | |
3.1.1.81 | ethyl paraoxon + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + diethyl phosphate | - |
? | |
3.1.8.1 | ethyl paraoxon + H2O | - |
Saccharolobus solfataricus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.81 | POX | - |
Saccharolobus solfataricus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.1.81 | additional information | - |
106°C is the melting temperature. The temperatures required to lose one half of enzymatic activity in 5 minutes are 92°C and 130°C in liquid and solid states respectively. More than 30% activity remains after autoclaving the enzyme powder at 121°C for 15 min | Saccharolobus solfataricus |
3.1.8.1 | additional information | - |
106°C is the melting temperature. The temperatures required to lose one half of enzymatic activity in 5 minutes are 92°C and 130°C in liquid and solid states, respectively. More than 30% activity remains after autoclaving the enzyme powder at 121°C for 15 min | Saccharolobus solfataricus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.1.81 | metabolism | the enzyme also exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents | Saccharolobus solfataricus |
3.1.8.1 | metabolism | the enzyme exhibits promiscuous phosphotriesterase activity for the degradation of organophosphorous chemicals including insecticides and chemical warfare agents | Saccharolobus solfataricus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.8.1 | 1.2 | - |
ethyl paraoxon | at pH 8.0 and 23°C | Saccharolobus solfataricus | |
3.1.8.1 | 1.4 | - |
ethyl paraoxon | with 50% (v/v) glycerol, at pH 8.0 and -18°C | Saccharolobus solfataricus | |
3.1.8.1 | 25 | - |
ethyl paraoxon | at pH 8.0 and 70°C | Saccharolobus solfataricus |