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Literature summary extracted from
- Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia (2015), Sci. Rep., 5, 8372 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.1.1.81 | sitting drop vapor diffusion method, X-ray structures of the enzyme bound to a fatty acid and to its cognate substrate N-decanoyl-L-homoserine lactone. The structures highlight possible structural determinants that may be involved in its extreme thermal stability | Vulcanisaeta moutnovskia |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.81 | 0.0021 | - |
gamma-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia |  |
| 3.1.1.81 | 0.047 | - |
gamma-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.105 | - |
delta-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.12 | - |
gamma-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.186 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.231 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.262 | - |
N-octanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.839 | - |
delta-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.872 | - |
gamma-heptalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 1.22 | - |
delta-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 3.709 | - |
Gamma-caprolactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.81 | Co2+ | bi-cobalt metalloenzyme. The two metal cations are coordinated by four histidines, an aspartic acid and a carboxylated lysine residue. Both of the metal cations are bridged by a putatively catalytic water molecule | Vulcanisaeta moutnovskia | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.81 | Vulcanisaeta moutnovskia | F0QXN6 | - |
- |
| 3.1.1.81 | Vulcanisaeta moutnovskia 768-28 | F0QXN6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.81 | - |
Vulcanisaeta moutnovskia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.81 | delta-dodecalactone + H2O | - |
Vulcanisaeta moutnovskia | 5-hydroxydodecanoic acid | - |
? | |
| 3.1.1.81 | delta-nonalactone + H2O | - |
Vulcanisaeta moutnovskia | 5-hydroxynonanoic acid | - |
? | |
| 3.1.1.81 | delta-nonalactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | 5-hydroxynonanoic acid | - |
? | |
| 3.1.1.81 | delta-undecalactone + H2O | - |
Vulcanisaeta moutnovskia | 5-hydroxyundecanoic acid | - |
? | |
| 3.1.1.81 | gamma-caprolactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxyhexanoic acid | - |
? | |
| 3.1.1.81 | gamma-dodecalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxydodecanoic acid | - |
? | |
| 3.1.1.81 | gamma-heptalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxyheptanoic acid | - |
? | |
| 3.1.1.81 | gamma-nonalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxynonanoic acid | - |
? | |
| 3.1.1.81 | gamma-nonalactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | 4-hydroxynonanoic acid | - |
? | |
| 3.1.1.81 | gamma-undecalactone + H2O | - |
Vulcanisaeta moutnovskia | 4-hydroxyundecanoic acid | - |
? | |
| 3.1.1.81 | additional information | VmoLac is a proficient lactonase with promiscuous, poor esterase and phosphotriesterase activities | Vulcanisaeta moutnovskia | ? | - |
? | |
| 3.1.1.81 | additional information | VmoLac is a proficient lactonase with promiscuous, poor esterase and phosphotriesterase activities | Vulcanisaeta moutnovskia 768-28 | ? | - |
? | |
| 3.1.1.81 | N-3-oxooctanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia | N-3-oxooctanoyl-L-homoserine | - |
? | |
| 3.1.1.81 | N-3-oxooctanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | N-3-oxooctanoyl-L-homoserine | - |
? | |
| 3.1.1.81 | N-decanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia | N-decanoyl-L-homoserine | - |
? | |
| 3.1.1.81 | N-decanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia 768-28 | N-decanoyl-L-homoserine | - |
? | |
| 3.1.1.81 | N-octanoyl-L-homoserine lactone + H2O | - |
Vulcanisaeta moutnovskia | N-octanoyl-L-homoserine | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.81 | VmoLac | - |
Vulcanisaeta moutnovskia |
| 3.1.1.81 | VMUT_2255 | - |
Vulcanisaeta moutnovskia |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.81 | 128 | - |
Tm-value | Vulcanisaeta moutnovskia |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.81 | 0.16 | - |
gamma-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.35 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.47 | - |
N-decanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 0.59 | - |
N-octanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 8.86 | - |
gamma-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 27.25 | - |
gamma-heptalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 28.08 | - |
delta-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 44.49 | - |
gamma-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 60.78 | - |
delta-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 88.91 | - |
delta-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 112.3 | - |
Gamma-caprolactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.81 | 1.88 | - |
N-3-oxooctanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 2.23 | - |
N-octanoyl-L-homoserine lactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 30.3 | - |
Gamma-caprolactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 31.1 | - |
gamma-heptalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 72.9 | - |
delta-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 73.6 | - |
gamma-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 77.7 | - |
gamma-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 335 | - |
delta-dodecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 580 | - |
delta-undecalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
| 3.1.1.81 | 948 | - |
gamma-nonalactone | pH and temperature not specified in the publication | Vulcanisaeta moutnovskia | |
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