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Literature summary extracted from

  • Dubrovskaya, Y.; Shubina, L.; Makarieva, T.; Bakunina, I.
    The effect of pentacyclic guanidine alkaloids from the marine sponge Monanchora pulchra Lambe, 1894 on the activity of natural beta-1,3-D-glucanase from the marine fungus Chaetomium indicum Corda, 1840 and the marine bivalve mollusk Spisula sachalinensis, S (2018), Russ. J. Mar. Biol., 44, 127-134 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.39 monanchocidin A activates to 364.1% activity at 0.257 mM Pseudocardium sachalinense
3.2.1.39 monanchomycalin B activates to 275.5% activity at 0.278 mM Pseudocardium sachalinense
3.2.1.39 additional information stimulating effects of pentacyclic guanidine alkaloids (monanchomycalin B, monanchocidin A, and normonanchocidin A) from the marine sponge Monanchora pulchra on endo-beta-1,3-D-glucanase LIV enzyme activity, compound identification by mass spectrometry Pseudocardium sachalinense
3.2.1.39 normonanchocidin A activates to 466.7% activity at 0.287 mM Pseudocardium sachalinense

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.58 monanchocidin A inhibits 98.6% at 0.257 mM, irreversible slow inhibition Dichotomopilus indicus
3.2.1.58 monanchomycalin B inhibits 98.5% at 0.278 mM, irreversible slow inhibition Dichotomopilus indicus
3.2.1.58 additional information inhibitory effects of pentacyclic guanidine alkaloids (monanchomycalin B, monanchocidin A, and normonanchocidin A) from the marine sponge Monanchora pulchra on exo-beta-1,3-D-glucanase ChinLam enzyme activity, compound identification by mass spectrosmetry Dichotomopilus indicus
3.2.1.58 normonanchocidin A inhibits 99.3% at 0.287 mM, irreversible slow inhibition Dichotomopilus indicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.58 additional information
-
 additional information Michaelis-Menten kinetics Dichotomopilus indicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.39 laminarin + H2O Pseudocardium sachalinense
-
 ?
-
 ?
3.2.1.58 laminarin + H2O Dichotomopilus indicus
-
 ?
-
 ?
3.2.1.58 additional information Dichotomopilus indicus exo-1,3-beta-D-glucanases remove the glucose residues or gentiobiose (Glc-beta-1,6-Glc) from the non-reducing ends of beta-1,3-D-glucans, beta-1,3-1,6-D-glucans and laminarioligosaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Pseudocardium sachalinense Q7Z0T2 i.e. Pseudocardium sachalinense
-
3.2.1.58 Dichotomopilus indicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Pseudocardium sachalinense ?
-
 ?
3.2.1.39 additional information endo-1,3-beta-D-glucanases catalyze the hydrolysis of internal beta-(1,3)-O-glycosidic bonds Pseudocardium sachalinense ?
-
 ?
3.2.1.58 laminarin + H2O
-
 Dichotomopilus indicus ?
-
 ?
3.2.1.58 additional information exo-1,3-beta-D-glucanases remove the glucose residues or gentiobiose (Glc-beta-1,6-Glc) from the non-reducing ends of beta-1,3-D-glucans, beta-1,3-1,6-D-glucans and laminarioligosaccharides Dichotomopilus indicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 beta-1,3-glucanase
-
 Pseudocardium sachalinense
3.2.1.39 endo-beta-1,3-D-glucanase
-
 Pseudocardium sachalinense
3.2.1.39 SpsLamIV
-
 Pseudocardium sachalinense
3.2.1.58 beta-1,3-glucanase
-
 Dichotomopilus indicus
3.2.1.58 ChinLam
-
 Dichotomopilus indicus
3.2.1.58 exo-beta-1,3-D-glucanase
-
 Dichotomopilus indicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 37
-
 assay at Pseudocardium sachalinense
3.2.1.58 37
-
 assay at Dichotomopilus indicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5.2
-
 assay at Pseudocardium sachalinense
3.2.1.58 5.2
-
 assay at Dichotomopilus indicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.58 additional information
-
 additional information inhibition kinetics, upon inhibition by monanchomycalin A and monanchocidin A, a positive cooperability is observed Dichotomopilus indicus
3.2.1.58 0.0135
-
 normonanchocidin A pH 5.2, 37°C Dichotomopilus indicus
3.2.1.58 0.0894
-
 monanchocidin A pH 5.2, 37°C Dichotomopilus indicus
3.2.1.58 0.2164
-
 monanchomycalin B pH 5.2, 37°C Dichotomopilus indicus