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Literature summary extracted from

  • Dalberto, P.; Martinelli, L.; Bachega, J.; Timmers, L.; Pinto, A.; Dadda, A.; Petersen, G.; Subtil, F.; Galina, L.; Villela, A.; Pissinate, K.; Machado, P.; Bizarro, C.; De Souza, O.; De Carvalho Filho, E.; Basso, L.; Santos, D.
    Thermodynamics, functional and structural characterization of inosine-uridine nucleoside hydrolase from Leishmania braziliensis (2017), RSC Adv., 7, 48861-48875 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.8 expression in Escherichia coli Leishmania braziliensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.2.8 to 1.53 A resolution, orthorhombic space group I222 Leishmania braziliensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.2.8 0.25
-
 4-nitrophenyl beta-D-ribofuranoside pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 0.5
-
 Inosine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 0.6
-
 adenosine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 0.7
-
 cytidine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 3.6
-
 uridine positive homotropic cooperativity, Hill coefficient value of 2.4, pH 7.5, 25°C Leishmania braziliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.8 136000
-
 gel filtration Leishmania braziliensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.8 Leishmania braziliensis A4H9Q9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.8 4-nitrophenyl beta-D-ribofuranoside + H2O
-
 Leishmania braziliensis 4-nitrophenol + D-ribose
-
 ?
3.2.2.8 adenosine + H2O
-
 Leishmania braziliensis adenine + D-ribose
-
 ?
3.2.2.8 cytidine + H2O
-
 Leishmania braziliensis cytosine + D-ribose
-
 ?
3.2.2.8 guanosine + H2O
-
 Leishmania braziliensis guanine + D-ribose
-
 ?
3.2.2.8 inosine + H2O
-
 Leishmania braziliensis D-ribose + hypoxanthine
-
 ?
3.2.2.8 uridine + H2O
-
 Leishmania braziliensis uracil + D-ribose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.8 tetramer 4 * 34000, SDS-PAGE, 4 * 34340, calculated from sequence Leishmania braziliensis

Synonyms

EC Number Synonyms Comment Organism
3.2.2.8 LBRM_18_1610
-
 Leishmania braziliensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.2.8 0.039
-
 adenosine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 0.066
-
 cytidine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 5.1
-
 uridine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 11.2
-
 Inosine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 344
-
 4-nitrophenyl beta-D-ribofuranoside pH 7.5, 25°C Leishmania braziliensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.2.8 0.047
-
 guanosine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 0.065
-
 adenosine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 0.094
-
 cytidine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 22
-
 Inosine pH 7.5, 25°C Leishmania braziliensis
3.2.2.8 1300
-
 4-nitrophenyl beta-D-ribofuranoside pH 7.5, 25°C Leishmania braziliensis