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Literature summary extracted from

  • Shufen, C.; Yicong, C.; Baobing, F.; Guiai, J.; Zhonghua, S.; Ju, L.; Shaoqing, T.; Jianlong, W.; Peisong, H.; Xiangjin, W.
    Editing of rice isoamylase gene ISA1 provides insights into its function in starch formation (2019), Rice Sci., 26, 77-87 .
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.68 amylopectin + H2O Oryza sativa Japonica Group
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Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Oryza sativa Japonica Group
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cultivar Zhonghua 11
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.68 endosperm
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Oryza sativa Japonica Group
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 amylopectin + H2O
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Oryza sativa Japonica Group ?
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?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.68 ISA1
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Oryza sativa Japonica Group
3.2.1.68 isoamylase 1
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Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
3.2.1.68 malfunction seeds of the homozygous mutants, cr-isa1-1 (type 1, with an adenine insertion) and cr-isa1-2 (type 3, with a cytosine deletion) display a shrunken endosperm with significantly lower grain weight. Abnormal starch granules and amyloplasts are found in cr-isa1-1 and cr-isa1-2 endosperm cells. The contents of total starch, amylose and amylopectin in the endosperm of the cr-isa1 mutants are significantly reduced, whereas sugar content and starch gel consistency were observably increased compared to the wild type. The gelatinization temperature and starch chain length distributions of the cr-isa1 mutants are also altered. The transcript levels of most starch synthesis-related genes are significantly lower in cr-isa1 mutants Oryza sativa Japonica Group