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Literature summary extracted from

  • Hantani, Y.; Motoki, S.; Miyagawa, A.; Yamamura, H.; Oda, M.
    Transglycosylation activity of catalytic domain mutant of endo-1,3-beta-glucanase from Cellulosimicrobium cellulans (2018), Protein Pept. Lett., 25, 734-739 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.39 E119G site-directed mutagenesis, glucosidase inactive catalytic residue mutant, the mutant shows transglycosylation activity toward laminarioligosaccharides. The hydrolytic as well as transglycosylation activities of E119G decrease with the decrease in temperature, but the ratio of transglycosylation products increases. The enzymatic activity of E119G toward laminaritriose in the presence of glucose is abolished, while the addition of laminaribiose evidently increases the transglycosylation products such as laminaritetraose and laminaripentaose Cellulosimicrobium cellulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Cellulosimicrobium cellulans P22222 i.e. Arthrobacter luteus
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3.2.1.39 Cellulosimicrobium cellulans DK-1 P22222 i.e. Arthrobacter luteus
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminaribiose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans ?
-
 ?
3.2.1.39 laminaribiose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans DK-1 ?
-
 ?
3.2.1.39 laminaritriose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans ?
-
 ?
3.2.1.39 laminaritriose + H2O wild-type enzyme performs glucosidase activity on laminarioligosaccharides, while the mutant performs transglycosylation Cellulosimicrobium cellulans DK-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 endo-1,3-beta-glucanase
-
 Cellulosimicrobium cellulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.39 4 8.5 activity range, lower activity at pH 8.0-8.5 Cellulosimicrobium cellulans

General Information

EC Number General Information Comment Organism
3.2.1.39 malfunction substitution of catalytic residue E119 in glycosidase results in the loss of hydrolytic activity Cellulosimicrobium cellulans
3.2.1.39 additional information residue Glu119 acts as a nucleophile in the endo-1,3-beta-glucanase reaction and has an effect on the balance between hydrolysis and transglycosylation Cellulosimicrobium cellulans