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Literature summary extracted from

  • Li, L.; Huang, C.; Zhao, F.; Deng, T.; Lin, Y.; Zheng, S.; Liang, S.; Han, S.
    Improved production and characterization of Volvariella volvacea endoglucanase 1 expressed in Pichia pastoris (2018), Protein Expr. Purif., 152, 107-113 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 DTT 24.1% activation at 10 mM Volvariella volvacea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 gene eg1, recombinant expression in Pichia pastoris strains GS115 and X33, enzyme EG1 folding and secretion efficiency from the endoplasmic reticulum is best enhanced by co-overexpression of chaperone HAC1 in the host strain GS115 harboring eight EG1 copies, subcloning in Escherichia coli strain TOP10, quantitative PCR expression analysis, method evaluation and optimization for high enzyme production Volvariella volvacea

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ca2+ 12.7% inhibition at 10 mM Volvariella volvacea
3.2.1.4 Co2+ 10.1% inhibition at 10 mM Volvariella volvacea
3.2.1.4 Cu2+ 37.7% inhibition at 10 mM Volvariella volvacea
3.2.1.4 Ni2+ 12.1% inhibition at 10 mM Volvariella volvacea
3.2.1.4 SDS 52.1% inhibition at 1% Volvariella volvacea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information KM is 14.3 mg/ml, substrate carboxymethyl cellulose, recombinant enzyme, pH 5.0, 60°C Volvariella volvacea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular the enzyme is secreted Volvariella volvacea
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ba2+ 26.1% activation at 10 mM Volvariella volvacea
3.2.1.4 K+ 32.8% activation at 10 mM Volvariella volvacea
3.2.1.4 Li+ 5.2% activation at 10 mM Volvariella volvacea
3.2.1.4 Mg2+ 5.1% activation at 10 mM Volvariella volvacea
3.2.1.4 Mn2+ 25.8% activation at 10 mM Volvariella volvacea
3.2.1.4 additional information no effect by Al3+ at 10 mM Volvariella volvacea
3.2.1.4 Zn2+ 10.0% activation at 10 mM Volvariella volvacea

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Volvariella volvacea B3GQ73
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Volvariella volvacea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 EG1
-
 Volvariella volvacea
3.2.1.4 endoglucanase 1
-
 Volvariella volvacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
 recombinant enzyme Volvariella volvacea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 74.51
-
 carboxymethyl cellulose recombinant enzyme, pH 5.0, 60°C Volvariella volvacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
 recombinant enzyme Volvariella volvacea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 additional information kcat/KM is 312.9 ml*mg/min, substrate carboxymethyl cellulose, recombinant enzyme, pH 5.0, 60°C Volvariella volvacea