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Literature summary extracted from

  • Tuntrakool, P.; Keawsompong, S.
    Kinetic properties analysis of beta-mannanase from Klebsiella oxytoca KUB-CW2-3 expressed in Escherichia coli (2018), Protein Expr. Purif., 146, 23-26 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Escherichia coli strain KMAN-3 Klebsiella oxytoca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Klebsiella oxytoca
-
-
-
3.2.1.78 Klebsiella oxytoca KUB-CW2-3
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 Klebsiella oxytoca

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 Ni-NTA agarose resin column chromatography Klebsiella oxytoca

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 240
-
 crude lysate, at pH 4.0 and 40°C Klebsiella oxytoca
3.2.1.78 11900
-
 after 51fold purification, at pH 4.0 and 40°C Klebsiella oxytoca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 defatted copra meal + H2O
-
 Klebsiella oxytoca ?
-
 ?
3.2.1.78 defatted copra meal + H2O
-
 Klebsiella oxytoca KUB-CW2-3 ?
-
 ?
3.2.1.78 ivory nut mannan + H2O lowest activity Klebsiella oxytoca ?
-
 ?
3.2.1.78 ivory nut mannan + H2O lowest activity Klebsiella oxytoca KUB-CW2-3 ?
-
 ?
3.2.1.78 konjac glucomannan + H2O highest activity Klebsiella oxytoca ?
-
 ?
3.2.1.78 konjac glucomannan + H2O highest activity Klebsiella oxytoca KUB-CW2-3 ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Klebsiella oxytoca ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Klebsiella oxytoca KUB-CW2-3 ?
-
 ?
3.2.1.78 additional information no activity with carboxymethyl cellulose Klebsiella oxytoca ?
-
 ?
3.2.1.78 additional information no activity with carboxymethyl cellulose Klebsiella oxytoca KUB-CW2-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 43000, SDS-PAGE Klebsiella oxytoca

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 Beta-mannanase
-
 Klebsiella oxytoca
3.2.1.78 endo-1,4-beta-mannanase
-
 Klebsiella oxytoca
3.2.1.78 KMAN-3 recombinant enzyme Klebsiella oxytoca