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Literature summary extracted from

  • Xu, Q.; Cao, Y.; Li, X.; Liu, L.; Qin, S.; Wang, Y.; Cao, Y.; Xu, H.; Qiao, D.
    Purification and characterization of a novel intracellular alpha-amylase with a wide variety of substrates hydrolysis and transglycosylation activity from Paenibacillus sp. SSG-1 (2018), Protein Expr. Purif., 144, 62-70 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Paenibacillus sp. SSG-1

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 2-mercaptoethanol 85.9% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Co2+ 75.9% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Cu2+ 64.1% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 DTT 20.1% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 EDTA 81.9% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Fe2+ 55.3% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Li+ 26.2% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Mn2+ 49.1% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Ni2+ 65.9% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Pb2+ 80.6% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 PMSF 21.2% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 SDS 71.0% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Urea 58.5% inhibition at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Zn2+ 80.5% inhibition at 1 mM Paenibacillus sp. SSG-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 0.13
-
 soluble starch pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 1.42
-
 maltoheptaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 1.92
-
 maltopentaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 2.28
-
 maltotriose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 2.86
-
 maltose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 3.25
-
 amylose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 cytoplasm
-
 Paenibacillus sp. SSG-1 5737
-
3.2.1.1 intracellular
-
 Paenibacillus sp. SSG-1 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ba2+ activates 7.4% at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Ca2+ activates 28.2% at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 Mg2+ activates 6.9% at 1 mM Paenibacillus sp. SSG-1
3.2.1.1 additional information poor effects by 1 mM of K+ and Na+ Paenibacillus sp. SSG-1

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Paenibacillus sp. SSG-1 A0A0U4VEK2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Paenibacillus sp. SSG-1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 122.04
-
 purified recombinant enzyme, pH 6.5, 40°C, substrate amylose Paenibacillus sp. SSG-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O 71.1% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
3.2.1.1 amylose + H2O best substrate Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
3.2.1.1 beta-cyclodextrin + H2O 39.0% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
3.2.1.1 glycogen + H2O 40.2% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
3.2.1.1 maltoheptaose + H2O
-
 Paenibacillus sp. SSG-1 D-glucose + maltose + ?
-
 ?
3.2.1.1 maltopentaose + H2O
-
 Paenibacillus sp. SSG-1 D-glucose + maltose + ?
-
 ?
3.2.1.1 maltose + H2O
-
 Paenibacillus sp. SSG-1 2 D-glucose
-
 ?
3.2.1.1 maltotriose + H2O
-
 Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
3.2.1.1 additional information the enzyme Amy can hydrolyze typical substrates into glucose and maltose, substrate specificity, overview. Enzyme Amy broad substrate range and transglycosylation activity when compared with intracellular alpha-amylase AmyB. Amy exhibits transglycosylation activity when reacted with maltose and maltotriose Paenibacillus sp. SSG-1 ?
-
 ?
3.2.1.1 pullulan + H2O 54.4% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?
3.2.1.1 soluble starch + H2O 93.3% activity compared to amylose Paenibacillus sp. SSG-1 D-glucose + maltose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 71700, recombinant His-tagged enzyme, SDS-PAGE Paenibacillus sp. SSG-1

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 Amy
-
 Paenibacillus sp. SSG-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 35 45 recombinant His-tagged enzyme Paenibacillus sp. SSG-1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1
-
 55 activity range, profile overview Paenibacillus sp. SSG-1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1
-
 40 purified recombinant His-tagged enzyme, over 80% activity remaining Paenibacillus sp. SSG-1
3.2.1.1 50
-
 purified recombinant His-tagged enzyme, 60% activity remaining Paenibacillus sp. SSG-1
3.2.1.1 60
-
 purified recombinant His-tagged enzyme, 10% activity remaining Paenibacillus sp. SSG-1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 0.72
-
 soluble starch pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 9.21
-
 amylose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 428.98
-
 maltose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 538.95
-
 maltotriose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 567.01
-
 maltopentaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 573.8
-
 maltoheptaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6.5
-
 recombinant His-tagged enzyme Paenibacillus sp. SSG-1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 5.6 8 50% of maximal activity of recombinant His-tagged enzyme within this range, inactive below pH 4.5 Paenibacillus sp. SSG-1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5
-
 purified recombinant His-tagged enzyme, 30% activity remaining Paenibacillus sp. SSG-1
3.2.1.1 5.5 7.5 purified recombinant His-tagged enzyme, 80% activity remaining Paenibacillus sp. SSG-1
3.2.1.1 9
-
 purified recombinant His-tagged enzyme, 30% activity remaining Paenibacillus sp. SSG-1

General Information

EC Number General Information Comment Organism
3.2.1.1 evolution enzyme Amy from Paenibacillus sp. SSG-1 exhibits unexpectedly sequence similarity and evolutionary relationships with alpha-glucanotransferase, homology modelling, using the crystal structure of a secretory alpha-amylase AmyA (PDB ID: 1WZA) as the template, and phylogenetic analysis Paenibacillus sp. SSG-1
3.2.1.1 additional information docking study of the enzyme with maltose Paenibacillus sp. SSG-1
3.2.1.1 physiological function enzyme Amy may play an important role in utilizing maltooligosaccharides that are released from extracellular alpha-glucan or storage alpha-glucan (glycogen) in Paenibacillus sp. SSG-1 Paenibacillus sp. SSG-1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.1 2.83
-
 amylose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 5.41
-
 soluble starch pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 150.17
-
 maltose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 236.38
-
 maltotriose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 295.32
-
 maltopentaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1
3.2.1.1 405.03
-
 maltoheptaose pH 6.5, 40°C, recombinant enzyme Paenibacillus sp. SSG-1