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Literature summary extracted from

  • An, K.; Shi, X.; Cui, F.; Cheng, J.; Liu, N.; Zhao, X.; Zhang, X.H.
    Characterization and overexpression of a glycosyl hydrolase family 16 beta-agarase YM01-1 from marine bacterium Catenovulum agarivorans YM01T (2018), Protein Expr. Purif., 143, 1-8 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 expressed in Escherichia coli BL21(DE3) cells Catenovulum agarivorans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 Co2+ 27.39% residual activity at 10 mM Catenovulum agarivorans
3.2.1.81 Cu2+ complete inhibition at 1 mM Catenovulum agarivorans
3.2.1.81 EDTA 26.75% residual activity at 10 mM Catenovulum agarivorans
3.2.1.81 Fe3+ complete inhibition at 10 mM Catenovulum agarivorans
3.2.1.81 Mn2+ complete inhibition at 10 mM Catenovulum agarivorans
3.2.1.81 Ni2+ 90.26% residual activity at 10 mM Catenovulum agarivorans
3.2.1.81 SDS 85.76% residual activity at 10 mM Catenovulum agarivorans
3.2.1.81 Urea 85.86% residual activity at 10 mM Catenovulum agarivorans
3.2.1.81 Zn2+ 35.79% residual activity at 10 mM Catenovulum agarivorans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.81 Ca2+ 110.56% activity at 10 mM Catenovulum agarivorans
3.2.1.81 K+ 120.36% activity at 10 mM Catenovulum agarivorans
3.2.1.81 Mg2+ 208.21% activity at 10 mM Catenovulum agarivorans
3.2.1.81 Na+ 112.65% activity at 10 mM Catenovulum agarivorans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Catenovulum agarivorans A0A286PT85
-
-
3.2.1.81 Catenovulum agarivorans YM01 A0A286PT85
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.81 nickel-chelating affinity column chromatography Catenovulum agarivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
Catenovulum agarivorans neoagarobiose
-
?
3.2.1.81 agarose + H2O
-
Catenovulum agarivorans YM01 neoagarobiose
-
?
3.2.1.81 neoagarodecaose + H2O
-
Catenovulum agarivorans neoagarobiose
-
?
3.2.1.81 neoagarodecaose + H2O
-
Catenovulum agarivorans YM01 neoagarobiose
-
?
3.2.1.81 neoagarohexaose + H2O
-
Catenovulum agarivorans neoagarobiose
-
?
3.2.1.81 neoagarohexaose + H2O
-
Catenovulum agarivorans YM01 neoagarobiose
-
?
3.2.1.81 neoagarooctaose + H2O
-
Catenovulum agarivorans neoagarobiose
-
?
3.2.1.81 neoagarooctaose + H2O
-
Catenovulum agarivorans YM01 neoagarobiose
-
?
3.2.1.81 neoagarotetraose + H2O
-
Catenovulum agarivorans neoagarobiose
-
?
3.2.1.81 neoagarotetraose + H2O
-
Catenovulum agarivorans YM01 neoagarobiose
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 ? x * 37700, calculated from amino acid sequence Catenovulum agarivorans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 AGA
-
Catenovulum agarivorans
3.2.1.81 endo-type beta-agarase
-
Catenovulum agarivorans
3.2.1.81 YM01-1
-
Catenovulum agarivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 50
-
-
Catenovulum agarivorans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.81 40 55 more than 80% of the maximum activity remain within 40-55°C Catenovulum agarivorans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 25 45 the enzyme is stable at temperatures below 45°C. The thermostability of the enzyme begins to decrease precipitously above 50°C, while less than 10% of maximum activity remain at 70°C Catenovulum agarivorans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.81 2400
-
agarose at pH 8.0 and 50°C Catenovulum agarivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 7
-
-
Catenovulum agarivorans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.81 6 9 more than 50% activity between pH 6.0 and 9.0 Catenovulum agarivorans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.81 6 9 more than 80% of the maximal activity is kept after preincubation at 4°C for 12 h at a pH ranging from 6.0 to 9.0 Catenovulum agarivorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.81 Catenovulum agarivorans calculated from amino acid sequence
-
4.7

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.81 2700
-
agarose at pH 8.0 and 50°C Catenovulum agarivorans