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Literature summary extracted from

  • Li, Z.; Ji, K.; Dong, W.; Ye, X.; Wu, J.; Zhou, J.; Wang, F.; Chen, Q.; Fu, L.; Li, S.; Huang, Y.; Cui, Z.
    Cloning, heterologous expression, and enzymatic characterization of a novel glucoamylase GlucaM from Corallococcus sp. strain EGB (2017), Protein Expr. Purif., 129, 122-127 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Corallococcus coralloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Ba2+ 18% activation at 1 mM, 25% inhibition at 5 mM Corallococcus coralloides
3.2.1.3 Co2+ complete inhibition at 1-5 mM Corallococcus coralloides
3.2.1.3 Cr3+ 62% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
3.2.1.3 Cu2+ 22% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
3.2.1.3 EDTA 22% inhibition at 10 mM Corallococcus coralloides
3.2.1.3 Fe2+ 27% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
3.2.1.3 Fe3+ 62% inhibition at 1 mM, complete inhibition at 5 mM Corallococcus coralloides
3.2.1.3 Mn2+ 34.5% inhibition at 1-5 mM Corallococcus coralloides
3.2.1.3 Ni2+ 44% inhibition at 1 mM, 70% at 5 mM Corallococcus coralloides
3.2.1.3 SDS 30% inhibition at 5 mg/ml Corallococcus coralloides
3.2.1.3 Triton X-100 20% inhibition at 20 mg/ml Corallococcus coralloides
3.2.1.3 Zn2+ 28% inhibition at 1 mM, 43% at 5 mM Corallococcus coralloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information Michaelis-Menten kinetics Corallococcus coralloides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Ba2+ 18% activation at 1 mM, 25% inhibition at 5 mM Corallococcus coralloides
3.2.1.3 Ca2+ 8.5% activation at 1 mM, 5.5% at 5 mM Corallococcus coralloides
3.2.1.3 K+ 34% activation at 1 mM, 31% at 5 mM Corallococcus coralloides
3.2.1.3 Mg2+ 50% activation at 1 mM, 8% at 5 mM Corallococcus coralloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Corallococcus coralloides
-
 ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.3 additional information low concentrations (10%) of methanol, ethanol, acetonitrile, and DMSO do not affect the enzyme's activity Corallococcus coralloides

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Corallococcus coralloides A0A0H3WKG6
-
-
3.2.1.3 Corallococcus coralloides CCTCC M2012528 A0A0H3WKG6
-
-
3.2.1.3 Corallococcus coralloides EGB A0A0H3WKG6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 recombinant His-tagged enzyme 50.2fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Corallococcus coralloides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 180
-
 purified recombinant enzyme, pH 7.0, 50°C Corallococcus coralloides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 alpha-cyclodextrin + H2O
-
 Corallococcus coralloides ?
-
 ?
3.2.1.3 amylopectin + H2O from potato Corallococcus coralloides ?
-
 ?
3.2.1.3 amylose + H2O from potato Corallococcus coralloides ?
-
 ?
3.2.1.3 amylose + H2O from potato Corallococcus coralloides CCTCC M2012528 ?
-
 ?
3.2.1.3 amylose + H2O from potato Corallococcus coralloides EGB ?
-
 ?
3.2.1.3 dextran + H2O
-
 Corallococcus coralloides ?
-
 ?
3.2.1.3 dextran + H2O
-
 Corallococcus coralloides CCTCC M2012528 ?
-
 ?
3.2.1.3 dextran + H2O
-
 Corallococcus coralloides EGB ?
-
 ?
3.2.1.3 dextrin + H2O
-
 Corallococcus coralloides ?
-
 ?
3.2.1.3 dextrin + H2O
-
 Corallococcus coralloides CCTCC M2012528 ?
-
 ?
3.2.1.3 dextrin + H2O
-
 Corallococcus coralloides EGB ?
-
 ?
3.2.1.3 glycogen + H2O
-
 Corallococcus coralloides ?
-
 ?
3.2.1.3 additional information substrate specificity, overview Corallococcus coralloides ?
-
 ?
3.2.1.3 additional information substrate specificity, overview Corallococcus coralloides CCTCC M2012528 ?
-
 ?
3.2.1.3 additional information substrate specificity, overview Corallococcus coralloides EGB ?
-
 ?
3.2.1.3 pullulan + H2O
-
 Corallococcus coralloides ?
-
 ?
3.2.1.3 pullulan + H2O
-
 Corallococcus coralloides CCTCC M2012528 ?
-
 ?
3.2.1.3 pullulan + H2O
-
 Corallococcus coralloides EGB ?
-
 ?
3.2.1.3 starch + H2O
-
 Corallococcus coralloides ?
-
 ?
3.2.1.3 starch + H2O soluble starch and raw starch from sweet potato and corn Corallococcus coralloides ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.3 ? x * 72920, about, sequence calculation Corallococcus coralloides

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GHF15 glucoamylase
-
 Corallococcus coralloides
3.2.1.3 GlucaM
-
 Corallococcus coralloides
3.2.1.3 glucoamylase
-
 Corallococcus coralloides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
-
 Corallococcus coralloides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 30 80 recombinant enzyme, activity range, profile overview Corallococcus coralloides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 30 80 recombinant enzyme GlucaM retains full activity after 1 h of incubation at below 60°C and retains about 60% of the original activity after 1 h at 70°C, but it loses 60% of the original activity after 1 h of incubation at 80°C Corallococcus coralloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 7
-
-
 Corallococcus coralloides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 3 10 recombinant enzyme, activity range, profile overview Corallococcus coralloides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.3 Corallococcus coralloides sequence calculation
-
 6.31