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Literature summary extracted from

  • Spano, D.; Pospiskova, K.; Safarik, I.; Pisano, M.B.; Pintus, F.; Floris, G.; Medda, R.
    Chitinase III in Euphorbia characias latex Purification and characterization (2015), Protein Expr. Purif., 116, 152-158 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 2
-
 4-nitrophenyl N-acetyl-D-glucosaminide at pH 5.0 and 40°C Euphorbia characias

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.14 Ca2+ 494% activity at 0.5 mM Euphorbia characias
3.2.1.14 Mg2+ 416% activity at 5 mM Euphorbia characias
3.2.1.14 additional information Cu2+, Mn2+, Ni2+, and Fe2+ do not influence the enzyme activity Euphorbia characias

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Euphorbia characias U5LKV8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 magnetic chitin affinity adsorbation and DEAE-cellulose column chromatography Euphorbia characias

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 latex
-
 Euphorbia characias
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl N-acetyl-D-glucosaminide + H2O
-
 Euphorbia characias 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?
3.2.1.14 chitobiose + H2O
-
 Euphorbia characias ?
-
 ?
3.2.1.14 chitotriose + H2O
-
 Euphorbia characias ?
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Euphorbia characias N-acetyl-beta-D-glucosamine + chitobiose + chitotriose
-
 ?
3.2.1.14 N-acetyl-beta-D-glucosamine + H2O
-
 Euphorbia characias ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 36500, SDS-PAGE Euphorbia characias

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 chitinase III
-
 Euphorbia characias
3.2.1.14 ELC
-
 Euphorbia characias

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
-
 Euphorbia characias

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 37 55 more than 50% activity between 37 and 55°C Euphorbia characias

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 40 70 the enzyme remains stable (more than 90% activity) after 10 min at 40-70°C. After 20 min at 40-50°C, the enzyme retains more than 90% activity, while at 60°C the enzyme activity drops to 50%. The enzyme is inactive after 30 min at 70°C Euphorbia characias

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 34
-
 4-nitrophenyl N-acetyl-D-glucosaminide at pH 5.0 and 40°C Euphorbia characias

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Euphorbia characias