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Literature summary extracted from

  • Choengpanya, K.; Arthornthurasuk, S.; Wattana-amorn, P.; Huang, W.T.; Plengmuankhae, W.; Li, Y.K.; Kongsaeree, P.T.
    Cloning, expression and characterization of beta-xylosidase from Aspergillus niger ASKU28 (2015), Protein Expr. Purif., 115, 132-140 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.37 degradation the enzyme is useful for degradation of lignocellulosic biomass in bioethanol production, pulp bleaching process and beverage industry Aspergillus niger

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expressed in Pichia pastoris strains Y11430 and KM71H as an N-terminal fusion protein with the alpha-mating factor signal sequence and a poly-histidine tag Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.39
-
 3,4-dinitrophenyl beta-D-xyloside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 0.66
-
 4-nitrophenyl beta-D-xyloside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 4.01
-
 4-nitrophenyl beta-D-glucoside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 4.54
-
 4-nitrophenyl alpha-L-arabinopyranoside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 30.8
-
 xylobiose at pH 4.0 and 70°C Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Aspergillus niger A0A023J5W7
-
-
3.2.1.37 Aspergillus niger ASKU28 A0A023J5W7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein the enzyme has 10 potential N-glycosylation sites Aspergillus niger

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 phenyl Sepharose column chromatography Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 74.4 76.5 crude enzyme, at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 125 140 after 1.68fold purification, at pH 4.0 and 70°C Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 3,4-dinitrophenyl beta-D-xyloside + H2O 48 activity compared to 4-nitrophenyl beta-D-xyloside Aspergillus niger 3,4-dinitrophenol + D-xylose
-
 ?
3.2.1.37 4-nitrophenyl alpha-L-arabinopyranoside + H2O 4.9% activity compared to 4-nitrophenyl beta-D-xyloside Aspergillus niger 4-nitrophenol + L-arabinopyranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucoside + H2O 0.7% activity compared to 4-nitrophenyl beta-D-xyloside Aspergillus niger 4-nitrophenol + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucoside + H2O 0.7% activity compared to 4-nitrophenyl beta-D-xyloside Aspergillus niger ASKU28 4-nitrophenol + D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xyloside + H2O 100% activity Aspergillus niger 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xyloside + H2O 100% activity Aspergillus niger ASKU28 4-nitrophenol + D-xylose
-
 ?
3.2.1.37 beechwood xylan + H2O
-
 Aspergillus niger xylobiose + ?
-
 ?
3.2.1.37 beechwood xylan + H2O
-
 Aspergillus niger ASKU28 xylobiose + ?
-
 ?
3.2.1.37 xylobiose + H2O 0.4% activity compared to 4-nitrophenyl beta-D-xyloside Aspergillus niger 2 D-xylose
-
 ?
3.2.1.37 xylobiose + H2O 0.4% activity compared to 4-nitrophenyl beta-D-xyloside Aspergillus niger ASKU28 2 D-xylose
-
 ?
3.2.1.37 xylotetraose + H2O
-
 Aspergillus niger xylotriose + beta-D-xylose
-
 ?
3.2.1.37 xylotetraose + H2O
-
 Aspergillus niger ASKU28 xylotriose + beta-D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 84500, calculated from amino acid sequence Aspergillus niger
3.2.1.37 ? x * 130000, glycosylated recombinant enzyme, calculated from amino acid sequence Aspergillus niger
3.2.1.37 ? x * 85000-90000, deglycosylated recombinant enzyme, calculated from amino acid sequence Aspergillus niger
3.2.1.37 ? x * 95100, recombinant enzyme as N-terminal fusion protein with the alpha-mating factor signal sequence and a poly-histidine tag, calculated from amino acid sequence Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 glycoside hydrolase family 3 beta-xylosidase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 70
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 30 60 the recombinant enzyme shows thermostability up to 60°C with more than 60% activity remaining after incubation at this temperature for 30 min Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 13.5
-
 4-nitrophenyl beta-D-glucoside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 67.8
-
 xylobiose at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 93.8
-
 3,4-dinitrophenyl beta-D-xyloside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 113
-
 4-nitrophenyl alpha-L-arabinopyranoside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 334
-
 4-nitrophenyl beta-D-xyloside at pH 4.0 and 70°C Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 4 4.5
-
 Aspergillus niger

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.37 2 6.5 the recombinant enzyme is stable up to pH 6.5, when incubated at 4°C for 4 h Aspergillus niger

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.37 2.2
-
 xylobiose at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 3.37
-
 4-nitrophenyl beta-D-glucoside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 24.9
-
 4-nitrophenyl alpha-L-arabinopyranoside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 242
-
 3,4-dinitrophenyl beta-D-xyloside at pH 4.0 and 70°C Aspergillus niger
3.2.1.37 504
-
 4-nitrophenyl beta-D-xyloside at pH 4.0 and 70°C Aspergillus niger