EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.37 | degradation | the enzyme is useful for degradation of lignocellulosic biomass in bioethanol production, pulp bleaching process and beverage industry | Aspergillus niger |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.37 | expressed in Pichia pastoris strains Y11430 and KM71H as an N-terminal fusion protein with the alpha-mating factor signal sequence and a poly-histidine tag | Aspergillus niger |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.37 | 0.39 | - |
3,4-dinitrophenyl beta-D-xyloside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 0.66 | - |
4-nitrophenyl beta-D-xyloside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 4.01 | - |
4-nitrophenyl beta-D-glucoside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 4.54 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 30.8 | - |
xylobiose | at pH 4.0 and 70°C | Aspergillus niger |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.37 | Aspergillus niger | A0A023J5W7 | - |
- |
3.2.1.37 | Aspergillus niger ASKU28 | A0A023J5W7 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.37 | glycoprotein | the enzyme has 10 potential N-glycosylation sites | Aspergillus niger |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.37 | phenyl Sepharose column chromatography | Aspergillus niger |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 74.4 | 76.5 | crude enzyme, at pH 4.0 and 70°C | Aspergillus niger |
3.2.1.37 | 125 | 140 | after 1.68fold purification, at pH 4.0 and 70°C | Aspergillus niger |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.37 | 3,4-dinitrophenyl beta-D-xyloside + H2O | 48 activity compared to 4-nitrophenyl beta-D-xyloside | Aspergillus niger | 3,4-dinitrophenol + D-xylose | - |
? | |
3.2.1.37 | 4-nitrophenyl alpha-L-arabinopyranoside + H2O | 4.9% activity compared to 4-nitrophenyl beta-D-xyloside | Aspergillus niger | 4-nitrophenol + L-arabinopyranose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-glucoside + H2O | 0.7% activity compared to 4-nitrophenyl beta-D-xyloside | Aspergillus niger | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-glucoside + H2O | 0.7% activity compared to 4-nitrophenyl beta-D-xyloside | Aspergillus niger ASKU28 | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-xyloside + H2O | 100% activity | Aspergillus niger | 4-nitrophenol + D-xylose | - |
? | |
3.2.1.37 | 4-nitrophenyl beta-D-xyloside + H2O | 100% activity | Aspergillus niger ASKU28 | 4-nitrophenol + D-xylose | - |
? | |
3.2.1.37 | beechwood xylan + H2O | - |
Aspergillus niger | xylobiose + ? | - |
? | |
3.2.1.37 | beechwood xylan + H2O | - |
Aspergillus niger ASKU28 | xylobiose + ? | - |
? | |
3.2.1.37 | xylobiose + H2O | 0.4% activity compared to 4-nitrophenyl beta-D-xyloside | Aspergillus niger | 2 D-xylose | - |
? | |
3.2.1.37 | xylobiose + H2O | 0.4% activity compared to 4-nitrophenyl beta-D-xyloside | Aspergillus niger ASKU28 | 2 D-xylose | - |
? | |
3.2.1.37 | xylotetraose + H2O | - |
Aspergillus niger | xylotriose + beta-D-xylose | - |
? | |
3.2.1.37 | xylotetraose + H2O | - |
Aspergillus niger ASKU28 | xylotriose + beta-D-xylose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.37 | ? | x * 84500, calculated from amino acid sequence | Aspergillus niger |
3.2.1.37 | ? | x * 130000, glycosylated recombinant enzyme, calculated from amino acid sequence | Aspergillus niger |
3.2.1.37 | ? | x * 85000-90000, deglycosylated recombinant enzyme, calculated from amino acid sequence | Aspergillus niger |
3.2.1.37 | ? | x * 95100, recombinant enzyme as N-terminal fusion protein with the alpha-mating factor signal sequence and a poly-histidine tag, calculated from amino acid sequence | Aspergillus niger |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.37 | glycoside hydrolase family 3 beta-xylosidase | - |
Aspergillus niger |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 70 | - |
- |
Aspergillus niger |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 30 | 60 | the recombinant enzyme shows thermostability up to 60°C with more than 60% activity remaining after incubation at this temperature for 30 min | Aspergillus niger |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.37 | 13.5 | - |
4-nitrophenyl beta-D-glucoside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 67.8 | - |
xylobiose | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 93.8 | - |
3,4-dinitrophenyl beta-D-xyloside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 113 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 334 | - |
4-nitrophenyl beta-D-xyloside | at pH 4.0 and 70°C | Aspergillus niger |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 4 | 4.5 | - |
Aspergillus niger |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.37 | 2 | 6.5 | the recombinant enzyme is stable up to pH 6.5, when incubated at 4°C for 4 h | Aspergillus niger |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.37 | 2.2 | - |
xylobiose | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 3.37 | - |
4-nitrophenyl beta-D-glucoside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 24.9 | - |
4-nitrophenyl alpha-L-arabinopyranoside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 242 | - |
3,4-dinitrophenyl beta-D-xyloside | at pH 4.0 and 70°C | Aspergillus niger | |
3.2.1.37 | 504 | - |
4-nitrophenyl beta-D-xyloside | at pH 4.0 and 70°C | Aspergillus niger |