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Literature summary extracted from
- Expression, purification, and characterization of human osteoclastic protein-tyrosine phosphatase catalytic domain in Escherichia coli (2015), Protein Expr. Purif., 107, 7-12 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.48 | DTT | activates | Homo sapiens |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.48 | gene PTPRE, located on chromosome 12p12-p13, recombinant expression of isolated C-terminally His6-tagged intracellular catalytic domain of PTP-oc (DELTAPTP-oc) in Escherichia coli strain BL21(DE3), method evaluation | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.3.48 | additional information | generation of the isolated intracellular catalytic domain of PTP-oc (DELTAPTP-oc) | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.48 | NaCl | inhibits the catalytic domain by 60% at 2 M | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.48 | additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| 3.1.3.48 | 0.8018 | - |
4-nitrophenyl phosphate | pH 7.0, 37°C, recombinant enzyme | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.48 | membrane | a structurally unique transmembrane protein tyrosine phosphatase | Homo sapiens | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O | Homo sapiens | - |
[a protein]-tyrosine + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.48 | Homo sapiens | P23469 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.48 | recombinant isolated His6-tagged intracellular catalytic domain of PTP-oc 6.8fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.48 | B-lymphocyte | - |
Homo sapiens | - |
| 3.1.3.48 | additional information | osteoclastic protein tyrosine phosphatase (PTP-oc) is mainly expressed in precursors of osteoclasts, including B lymphocytes, cells of the monocyte-macrophage lineage, and mature osteoclasts | Homo sapiens | - |
| 3.1.3.48 | osteoclast | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.48 | 15700 | - |
purified recombinant catalytic domain, pH 7.0, 37°C, substrate 4-nitrophenyl phosphate | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.48 | 4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
| 3.1.3.48 | [a protein]-tyrosine phosphate + H2O | - |
Homo sapiens | [a protein]-tyrosine + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.48 | More | osteoclastic protein tyrosine phosphatase (PTP-oc) is a structurally unique transmembrane protein tyrosine phosphatase (PTP) that contains only a relatively small intracellular PTP catalytic domain, does not have an extracellular domain, and lacks a signal peptide proximal to the N-terminus | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.48 | osteoclastic protein-tyrosine phosphatase | - |
Homo sapiens |
| 3.1.3.48 | PTP | - |
Homo sapiens |
| 3.1.3.48 | PTP-oc | - |
Homo sapiens |
| 3.1.3.48 | PTPRE | - |
Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.48 | 37 | - |
recombinant intracellular catalytic domain of PTP-oc (DELTAPTP-oc) | Homo sapiens |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.48 | 26 | 42 | over 50% of maximal activity within this range, recombinant catalytic enzyme domain, profile overview | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.48 | 7 | - |
recombinant intracellular catalytic domain of PTP-oc (DELTAPTP-oc) | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.48 | 4 | 9 | activity range of the recombinant catalytic enzyme domain, profile overview | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.1.3.48 | evolution | osteoclastic protein tyrosine phosphatase (PTP-oc) belongs to the receptor PTP family | Homo sapiens |
| 3.1.3.48 | additional information | osteoclastic protein tyrosine phosphatase (PTP-oc) is a structurally unique transmembrane protein tyrosine phosphatase (PTP) that contains only a relatively small intracellular PTP catalytic domain, does not have an extracellular domain, and lacks a signal peptide proximal to the N-terminus | Homo sapiens |
| 3.1.3.48 | physiological function | osteoclastic protein tyrosine phosphatase (PTP-oc) is an activator of osteoclast differentiation and activity | Homo sapiens |
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