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Literature summary extracted from

  • Nakabayashi, M.; Kamachi, S.; Malle, D.; Yanamoto, T.; Kishishita, S.; Fujii, T.; Inoue, H.; Ishikawa, K.
    Construction of thermostable cellobiohydrolase I from the fungus Talaromyces cellulolyticus by protein engineering (2019), Protein Eng. Des. Sel., 32, 33-40 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.91 A401P the mutant enzyme shows improved thermostability compared to the wild type enzyme Talaromyces pinophilus
3.2.1.91 A426P the mutant shows 86.9% of wild type activity Talaromyces pinophilus
3.2.1.91 S273P the mutant enzyme shows improved thermostability compared to the wild type enzyme Talaromyces pinophilus
3.2.1.91 S298P the mutant shows 88% of wild type activity Talaromyces pinophilus
3.2.1.91 T237G the mutant enzyme shows improved thermostability compared to the wild type enzyme Talaromyces pinophilus
3.2.1.91 T262G the mutant shows 94.4% of wild type activity Talaromyces pinophilus
3.2.1.91 T262G /S298P /A426P the mutant shows 95.7% of wild type activity Talaromyces pinophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Talaromyces pinophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Talaromyces pinophilus 4-nitrophenol + beta-D-lactopyranose
-
 ?
3.2.1.91 avicel + H2O
-
 Talaromyces pinophilus ?
-
 ?
3.2.1.91 crystalline cellulose + H2O
-
 Talaromyces pinophilus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CBHI
-
 Talaromyces pinophilus
3.2.1.91 cellobiohydrolase I
-
 Talaromyces pinophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 65
-
-
 Talaromyces pinophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 63
-
 the melting temperature is 63°C. The wild type enzyme is inactive after 30 min at 65°C Talaromyces pinophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 5
-
-
 Talaromyces pinophilus