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Literature summary extracted from

  • Sharma, K.; Antunes, I.; Rajulapati, V.; Goyal, A.
    Molecular characterization of a first endo-acting beta-1,4-xylanase of family 10 glycoside hydrolase (PsGH10A) from Pseudopedobacter saltans comb. nov (2018), Process Biochem., 70, 79-89 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.8 additional information the enzyme can be used for various biotechnological applications such as for prebiotic xylooligosaccharides and bioethanol production from pretreated agrowaste biomass Pseudopedobacter saltans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 gene Pedsa_2704, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain TOP10 Pseudopedobacter saltans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ complete inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 Ba2+ 42% inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 Co2+ 39% inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 Cu2+ 85% inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 EDTA 36% inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 guanidine hydrochloride 84% inhibition at 2 M Pseudopedobacter saltans
3.2.1.8 Hg2+ complete inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 Li+ 54% inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 Ni2+ 48% inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 Pb2+ 65% inhibition at 2 mM Pseudopedobacter saltans
3.2.1.8 SDS 55% inhibition at 0.25% w/v Pseudopedobacter saltans
3.2.1.8 Urea 80% inhibition at 0.5 M Pseudopedobacter saltans
3.2.1.8 Zn2+ 51% inhibition at 2 mM Pseudopedobacter saltans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information Michaelis-Menten and Lineweaver-Burk kinetics, Km for beechwood xylan is 6.2 mg/ml for birchwood 3.9 mg/ml, and for xylan MW 2000-30000 5.1 mg/ml Pseudopedobacter saltans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Mn2+ activates by 29% at 2 mM Pseudopedobacter saltans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Pseudopedobacter saltans F0S6Y3 i.e. Pedobacter saltans
-
3.2.1.8 Pseudopedobacter saltans ATCC 51119 F0S6Y3 i.e. Pedobacter saltans
-
3.2.1.8 Pseudopedobacter saltans DSM 12145 F0S6Y3 i.e. Pedobacter saltans
-
3.2.1.8 Pseudopedobacter saltans JCM 21818 F0S6Y3 i.e. Pedobacter saltans
-
3.2.1.8 Pseudopedobacter saltans LMG 10337 F0S6Y3 i.e. Pedobacter saltans
-
3.2.1.8 Pseudopedobacter saltans NBRC 100064 F0S6Y3 i.e. Pedobacter saltans
-
3.2.1.8 Pseudopedobacter saltans NCIMB 13643 F0S6Y3 i.e. Pedobacter saltans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Pseudopedobacter saltans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 8
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate oat spelt xylan Pseudopedobacter saltans
3.2.1.8 12.7
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate insoluble wheat arabinoxylan Pseudopedobacter saltans
3.2.1.8 18.7
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate insoluble rye arabinoxylan Pseudopedobacter saltans
3.2.1.8 22.3
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate soluble wheat arabinoxylan Pseudopedobacter saltans
3.2.1.8 25.4
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate soluble rye arabinoxylan Pseudopedobacter saltans
3.2.1.8 37.3
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate birchwood xylan Pseudopedobacter saltans
3.2.1.8 47
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate 4-O methyl-glucuronoxylan Pseudopedobacter saltans
3.2.1.8 57.1
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate xylan with MW of 20000-30000 Pseudopedobacter saltans
3.2.1.8 59.7
-
 purified recombinant His-tagged enzyme, pH 6.0, 40°C, substrate beechwood xylan Pseudopedobacter saltans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 4-O methyl-glucuronoxylan + H2O moderate activity Pseudopedobacter saltans ?
-
 ?
3.2.1.8 beechwood xylan + H2O best substrate Pseudopedobacter saltans xylose + xylobiose + xylotetraose
-
 ?
3.2.1.8 birchwood xylan + H2O moderate activity Pseudopedobacter saltans ?
-
 ?
3.2.1.8 birchwood xylan + H2O moderate activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
3.2.1.8 birchwood xylan + H2O moderate activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
3.2.1.8 birchwood xylan + H2O moderate activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
3.2.1.8 birchwood xylan + H2O moderate activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
3.2.1.8 birchwood xylan + H2O moderate activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
3.2.1.8 birchwood xylan + H2O moderate activity Pseudopedobacter saltans DSM 12145 ?
-
 ?
3.2.1.8 additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans ?
-
 ?
3.2.1.8 additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans NBRC 100064 ?
-
 ?
3.2.1.8 additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans JCM 21818 ?
-
 ?
3.2.1.8 additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
3.2.1.8 additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans ATCC 51119 ?
-
 ?
3.2.1.8 additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans LMG 10337 ?
-
 ?
3.2.1.8 additional information enzyme PsGH10A hydrolyzes products of xylan and substituted xylan indicate production of series of short-chain xylooligosaccharides and arabinoxylo-saccharides, substrate specificity, overview Pseudopedobacter saltans DSM 12145 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O low activity Pseudopedobacter saltans ?
-
 ?
3.2.1.8 oat spelt xylan + H2O low activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O low activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O low activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O low activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O low activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
3.2.1.8 oat spelt xylan + H2O low activity Pseudopedobacter saltans DSM 12145 ?
-
 ?
3.2.1.8 rye arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
3.2.1.8 wheat arabinoxylan + H2O soluble and insoluble, the latter is less active, moderate to lower activity Pseudopedobacter saltans DSM 12145 ?
-
 ?
3.2.1.8 xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans ?
-
 ?
3.2.1.8 xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans NBRC 100064 ?
-
 ?
3.2.1.8 xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans JCM 21818 ?
-
 ?
3.2.1.8 xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans NCIMB 13643 ?
-
 ?
3.2.1.8 xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans ATCC 51119 ?
-
 ?
3.2.1.8 xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans LMG 10337 ?
-
 ?
3.2.1.8 xylan + H2O molecular weight of 20000-30000, high activity Pseudopedobacter saltans DSM 12145 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 43000, recombinant His-tagged enzyme, SDS-PAGE Pseudopedobacter saltans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 beta-1,4-xylanase
-
 Pseudopedobacter saltans
3.2.1.8 endo-acting beta-1,4-xylanase
-
 Pseudopedobacter saltans
3.2.1.8 Pedsa_2704
-
 Pseudopedobacter saltans
3.2.1.8 PsGH10A
-
 Pseudopedobacter saltans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 40
-
-
 Pseudopedobacter saltans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 10 55 recombinant enzyme, activity range, profile overview Pseudopedobacter saltans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 40
-
 purified recombinant enzyme, pH 6.0, 90 min, stable up to Pseudopedobacter saltans
3.2.1.8 45
-
 purified recombinant enzyme, pH 6.0, 90 min, loss of 50% activity Pseudopedobacter saltans
3.2.1.8 50
-
 purified recombinant enzyme, pH 6.0, 90 min, loss of 70% activity Pseudopedobacter saltans
3.2.1.8 60
-
 purified recombinant enzyme, pH 6.0, 90 min, inactivation Pseudopedobacter saltans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 36.8
-
 birchwood xylan His-tagged enzyme, pH 6.0, 40°C Pseudopedobacter saltans
3.2.1.8 45.9
-
 xylan His-tagged enzyme, pH 6.0, 40°C, substrate xylan MW 20000-30000 Pseudopedobacter saltans
3.2.1.8 52.2
-
 beechwood xylan His-tagged enzyme, pH 6.0, 40°C Pseudopedobacter saltans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 6
-
-
 Pseudopedobacter saltans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 5 9 recombinant enzyme, activity range, profile overview Pseudopedobacter saltans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 4.5
-
 purified recombinant enzyme, 25°C, 90 min, 25% activity remaining Pseudopedobacter saltans
3.2.1.8 5.5
-
 purified recombinant enzyme, 25°C, 90 min, 50% activity remaining Pseudopedobacter saltans
3.2.1.8 6 8 purified recombinant enzyme, 25°C, 90 min, over 80% activity remaining Pseudopedobacter saltans
3.2.1.8 8.5
-
 purified recombinant enzyme, 25°C, 90 min, 50% activity remaining Pseudopedobacter saltans
3.2.1.8 9
-
 purified recombinant enzyme, 25°C, 90 min, 25% activity remaining Pseudopedobacter saltans

General Information

EC Number General Information Comment Organism
3.2.1.8 evolution the enzyme belongs to the glycoside hydrolase family 10, GH10 Pseudopedobacter saltans