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Literature summary extracted from

  • Yang, S.; Liu, M.; Long, L.; Zhang, R.; Ding, S.
    Characterization of a cutinase from Myceliophthora thermophila and its application in polyester hydrolysis and deinking process (2018), Process Biochem., 66, 106-112 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.74 degradation enzyme shows an ink removal efficiency of 78.4% on laser-printed paper and 81.3% on newspaper at 30°C Thermothelomyces thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.74 expression in Pichia pastoris Thermothelomyces thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 Cu2+ 1 mM, 82% residual activity Thermothelomyces thermophilus
3.1.1.74 Ni2+ 1 mM, 74% residual activity Thermothelomyces thermophilus
3.1.1.74 SDS 1 mM, 16% residual activity Thermothelomyces thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.74 1.41
-
 4-nitrophenyl propionate pH 8.5, 30°C Thermothelomyces thermophilus
3.1.1.74 1.61
-
 4-nitrophenyl pentanoate pH 8.5, 30°C Thermothelomyces thermophilus
3.1.1.74 2.34
-
 4-nitrophenyl butanoate pH 8.5, 30°C Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Thermothelomyces thermophilus G2QH51
-
-
3.1.1.74 Thermothelomyces thermophilus DSM 1799 G2QH51
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl butanoate + H2O
-
 Thermothelomyces thermophilus 4-nitrophenol + butanoate
-
 ?
3.1.1.74 4-nitrophenyl butanoate + H2O
-
 Thermothelomyces thermophilus DSM 1799 4-nitrophenol + butanoate
-
 ?
3.1.1.74 4-nitrophenyl pentanoate + H2O 53% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus 4-nitrophenol + pentanoate
-
 ?
3.1.1.74 4-nitrophenyl pentanoate + H2O 53% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus DSM 1799 4-nitrophenol + pentanoate
-
 ?
3.1.1.74 4-nitrophenyl propionate + H2O 35% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus 4-nitrophenol + propionate
-
 ?
3.1.1.74 4-nitrophenyl propionate + H2O 35% of the activity with 4-nitrophenyl butanoate Thermothelomyces thermophilus DSM 1799 4-nitrophenol + propionate
-
 ?
3.1.1.74 cutin + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.1.1.74 cutin + H2O
-
 Thermothelomyces thermophilus DSM 1799 ?
-
 ?
3.1.1.74 poly(caprolactone) + H2O
-
 Thermothelomyces thermophilus ?
-
 ?
3.1.1.74 poly(caprolactone) + H2O
-
 Thermothelomyces thermophilus DSM 1799 ?
-
 ?
3.1.1.74 polyvinyl acetate + H2O substrate used in papermaking and in synthetic toner or ink Thermothelomyces thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.74 ? x * 23400, calculated from sequence, x * 23400, SDS-PAGE Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.74 MYCTH_2110987
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.74 30
-
-
 Thermothelomyces thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.74 25 30 more than 60% of maximum activity Thermothelomyces thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.74 25
-
 1.5 h, 80% residual activity Thermothelomyces thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.74 8.5
-
-
 Thermothelomyces thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.74 3 11 more than 60% of maximum activity Thermothelomyces thermophilus