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Literature summary extracted from
- Parameters optimization and kinetics study of alpha-amylase enzyme of Bacillus sp. MB6 isolated from vegetable waste (2017), Process Biochem., 52, 123-129 .
No PubMed abstract available
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | additional information | enzyme immobilization, best at 4% alginate as compared to agar agar and other concentrations of alginate, overview | Bacillus sp. MB6 |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | additional information | - |
additional information | Michaelis-Menten kinetics, Km is 5.45 mg/ml and Vmax is 24.15 mg/ml/min | Bacillus sp. MB6 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | starch + H2O | Bacillus sp. MB6 | - |
? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Bacillus sp. MB6 | - |
isolated from vegetable waste sample, which has been collected from the local vegetable market of Raipur, Chhattisgarh, India | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | native extracellular enzyme 1.63fold by ammonium sulfate fractionation and dialysis | Bacillus sp. MB6 |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | additional information | optimization of the enzyme production method, overview. Best at pH 6.0, 37°C for 48 h | Bacillus sp. MB6 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 270.59 | - |
immobilized enzyme, pH 6.0, 55°C | Bacillus sp. MB6 |
| 3.2.1.1 | 402.02 | - |
partially purified enzyme, pH 6.0, 55°C | Bacillus sp. MB6 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | starch + H2O | - |
Bacillus sp. MB6 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 53000, SDS-PAGE | Bacillus sp. MB6 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 55 | - |
- |
Bacillus sp. MB6 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6 | - |
- |
Bacillus sp. MB6 |
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