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Literature summary extracted from

  • Eom, G.; Oh, J.; Park, J.; Jegal, J.; Song, J.
    Secretory production of enzymatically active endo-beta-1,4-mannanase from Bacillus subtilis by ABC exporter in Escherichia coli (2016), Process Biochem., 51, 999-1005 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Escherichia coli XL10-Gold cells Bacillus subtilis

General Stability

EC Number General Stability Organism
3.2.1.78 the fused signal sequence of metalloprotease PrtA does not affect the enzymatic properties of the enzyme Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Bacillus subtilis
-
-
-
3.2.1.78 Bacillus subtilis WL7
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 Bacillus subtilis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Q-Sepharose column chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 locust bean gum + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.78 locust bean gum + H2O
-
 Bacillus subtilis WL7 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-beta-1,4-mannanase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 55
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 45 60 about 60% activity at 45°C, about 80% activity at 50°C, 100% activity at 55°C, about 80% activity at 60°C, about 20% activity at 65°C Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 35 45 the relatively high thermostability is retained after 1 h in the temperature range of 35-45°C and the stability rapidly decreases above 60°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 5 8 about 30% activity at pH 4.0, about 90% activity at pH 5.0, 100% activity at pH 6.0, about 90% activity at pH 7.0, about 70% activity at pH 8.0, about 20% activity at pH 9.0 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 6 9 the enzyme is highly stable with 100% activity remaining after 1 h within a pH range of 6.0-9.0 and about 80-90% activity remaining after 1 h within a pH range of 4.0-5.0. No residual activity is detected at pH 3.0 Bacillus subtilis