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Literature summary extracted from

  • Li, F.; Zhang, C.; Liu, Y.; Liu, D.; Xia, H.; Chen, S.
    Efficient production of (R)-3-hydroxybutyric acid by Pseudomonas sp. DS1001a and its extracellular poly(3-hydroxybutyrate) depolymerase (2016), Process Biochem., 51, 369-373 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.75 degradation About 8.58 g/l (R)-3-hydroxybutanoate is obtained after 8 h of incubation using extracellular PHB. The optimal conditions are 50°C and pH 8. Presence of CaCO3 increases (R)-3-hydroxybutanoate yields to 23.97 g/l by stabilizing the pH of the reaction system Pseudomonas sp. DS1001a

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.75 extracellular
-
 Pseudomonas sp. DS1001a
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.75 Pseudomonas sp. DS1001a
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.75 poly (3-hydroxybutanoate) n + n H2O
-
 Pseudomonas sp. DS1001a poly (3-hydroxybutanoate) n-1 + (R)-3-hydroxybutanoate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.75 50
-
-
 Pseudomonas sp. DS1001a

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.75 8
-
-
 Pseudomonas sp. DS1001a