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Literature summary extracted from

  • Liu, H.; Gong, J.; Li, H.; Lu, Z.; Li, H.; Qian, J.; Xu, Z.; Shi, J.
    Biochemical characterization and cloning of an endo-1,4-beta-mannanase from Bacillus subtilis YH12 with unusually broad substrate profile (2015), Process Biochem., 50, 712-721 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.78 2-mercaptoethanol 123.7% activity at 1 mM Bacillus subtilis
3.2.1.78 Tween 20 109.9% activity at 1 mM Bacillus subtilis
3.2.1.78 Tween 40 125.4% activity at 1 mM Bacillus subtilis
3.2.1.78 Tween 60 106.8% activity at 1 mM Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Escherichia coli JM109 cells Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 35.7% residual activity at 5 mM Bacillus subtilis
3.2.1.78 Al3+ 84.7% residual activity at 5 mM Bacillus subtilis
3.2.1.78 Cu2+ 95.2% residual activity at 5 mM Bacillus subtilis
3.2.1.78 dithiothreitol 85.5% residual activity at 5 mM Bacillus subtilis
3.2.1.78 EDTA 26.8% residual activity at 5 mM Bacillus subtilis
3.2.1.78 Fe3+ 80.9% residual activity at 1 mM Bacillus subtilis
3.2.1.78 Li+ 96.3% residual activity at 1 mM Bacillus subtilis
3.2.1.78 Mn2+ 89.6% residual activity at 5 mM Bacillus subtilis
3.2.1.78 phenylmethylsulfonyl fluoride 80.3% residual activity at 5 mM Bacillus subtilis
3.2.1.78 SDS 79.7% residual activity at 5 mM Bacillus subtilis
3.2.1.78 Zn2+ 95% residual activity at 5 mM Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.78 Ba2+ 228.3% activity at 1 mM Bacillus subtilis
3.2.1.78 Co2+ 120.7% activity at 1 mM Bacillus subtilis
3.2.1.78 K+ 119.8% activity at 1 mM Bacillus subtilis
3.2.1.78 Mg2+ 137.1% activity at 1 mM Bacillus subtilis
3.2.1.78 Na+ 121.9% activity at 1 mM Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Bacillus subtilis
-
-
-
3.2.1.78 Bacillus subtilis YH12
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.78 glycoprotein
-
 Bacillus subtilis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 ammonium sulfate precipitation, DEAE column chromatography and Sephadex G75 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 carrageenan gum + H2O 71% activity compared to locust bean gum Bacillus subtilis ?
-
 ?
3.2.1.78 carrageenan gum + H2O 71% activity compared to locust bean gum Bacillus subtilis YH12 ?
-
 ?
3.2.1.78 ceratonia siliqua gum + H2O 46.6% activity compared to locust bean gum Bacillus subtilis ?
-
 ?
3.2.1.78 fenugreek gum + H2O 268.1% activity compared to locust bean gum Bacillus subtilis ?
-
 ?
3.2.1.78 galactomannan + H2O the enzyme exhibits higher activity on galactomannan branched with (1->6)-linked alpha-D-galactose than glucomannan Bacillus subtilis ?
-
 ?
3.2.1.78 galactomannan + H2O the enzyme exhibits higher activity on galactomannan branched with (1->6)-linked alpha-D-galactose than glucomannan Bacillus subtilis YH12 ?
-
 ?
3.2.1.78 glucomannan + H2O
-
 Bacillus subtilis ?
-
 ?
3.2.1.78 glucomannan + H2O
-
 Bacillus subtilis YH12 ?
-
 ?
3.2.1.78 guar gum + H2O 350% activity compared to locust bean gum Bacillus subtilis ?
-
 ?
3.2.1.78 guar gum + H2O 350% activity compared to locust bean gum Bacillus subtilis YH12 ?
-
 ?
3.2.1.78 konjac powder + H2O 85.1% activity compared to locust bean gum Bacillus subtilis mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose
-
 ?
3.2.1.78 locust bean gum + H2O 100% activity Bacillus subtilis mannose + mannobiose + mannotriose + mannotetraose + mannopentaose + mannohexaose + mannoheptaose
-
 ?
3.2.1.78 additional information no activity on starch and xylan Bacillus subtilis ?
-
 ?
3.2.1.78 additional information no activity on starch and xylan Bacillus subtilis YH12 ?
-
 ?
3.2.1.78 xanthan gum + H2O 345.6% activity compared to locust bean gum Bacillus subtilis mannose + mannobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 40000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-1,4-beta-mannanase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 55
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.78 40 60 more than 80% activity between 40 and 60°C, less than 10% activity at 65°C Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 40 60 the enzyme is stable up to 60°C and retains 85% of its activity after 1.0 h of incubation Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6.5
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 3 8 more than 60% activity between pH 3.0 and 8.0, no activity at pH 10.0 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.78 3 9 after 1 h incubation at pH 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0, the enzyme retains about 68%, 82%, 93%, 95%, 90%, 68%, and 42% activity, respectively Bacillus subtilis