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Literature summary extracted from

  • Tao, W.; Xu, Q.; Huang, H.; Li, S.
    Efficient production of peracetic acid in aqueous solution with cephalosporin-deacetylating acetyl xylan esterase from Bacillus subtilis (2015), Process Biochem., 50, 2121-2127 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.41 expression in Escherichia coli Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.41 synthesis synthesis of peracetic acid. Recombinant enzyme can be efficiently produced in a low-cost autoinduction medium with an activity of 6800 U/ml. Optimal synthesis of peracetic is achieved with 0.30 mg/ml crude enzyme, 300 mM glycerol triacetate, and 1 M hydrogen peroxide, pH 8.0, and 2°C, which produces approximately 150 mM of peracetic acid within 5 min. When immobilized on an acrylate amino resin, the activity of the immobilized enzyme is 383.7 U/g Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.41 ethanol product inhibition Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.41 1.1
-
 H2O2 free enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 1.15
-
 H2O2 immobilized enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 140.7
-
 glycerol triacetate free enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 315.3
-
 glycerol triacetate immobilized enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 409.1
-
 ethyl acetate free enzyme, pH 6.5, 25°C Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.41 Bacillus subtilis M1JUH6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.41 ethyl acetate + H2O2
-
 Bacillus subtilis ethanol + peracetic acid
-
 ?
3.1.1.41 glycerol triacetate + H2O2
-
 Bacillus subtilis glycerol diacetate + peracetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.41 ? x * 35600, SDS-PAGE Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.41 0.089
-
 ethyl acetate free enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 0.4
-
 glycerol triacetate immobilized enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 1.09
-
 H2O2 immobilized enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 2.53
-
 glycerol triacetate free enzyme, pH 6.5, 25°C Bacillus subtilis
3.1.1.41 3.3
-
 H2O2 free enzyme, pH 6.5, 25°C Bacillus subtilis