EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.15.36 | expression in Escherichia coli | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.36 | cytosol | - |
Mycobacterium tuberculosis | 5829 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.36 | Mycobacterium tuberculosis | P9WPP9 | - |
- |
1.14.15.36 | Mycobacterium tuberculosis H37Rv | P9WPP9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.36 | 24,25-dihydrolanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycobacterium tuberculosis | 4,4-dimethyl-5alpha-cholesta-8,14-diene-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
1.14.15.36 | 24,25-dihydrolanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycobacterium tuberculosis H37Rv | 4,4-dimethyl-5alpha-cholesta-8,14-diene-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
1.14.15.36 | lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycobacterium tuberculosis | 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
1.14.15.36 | lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycobacterium tuberculosis H37Rv | 4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
1.14.15.36 | additional information | both flavodoxin and ferredoxin redox systems are able to support the enzymatic activity. Substrates require a 14alpha methyl group and a 8,9 C-C double bond | Mycobacterium tuberculosis | ? | - |
? | |
1.14.15.36 | additional information | both flavodoxin and ferredoxin redox systems are able to support the enzymatic activity. Substrates require a 14alpha methyl group and a 8,9 C-C double bond | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
1.14.15.36 | obtusifoliol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycobacterium tuberculosis | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? | |
1.14.15.36 | obtusifoliol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 | - |
Mycobacterium tuberculosis H37Rv | 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.15.36 | ? | x * 50000, SDS-PAGE, x * 51400, calculated from sequence | Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.36 | cytochrome P450 | the oxidized absolute spectrum of the purified enzyme, in the absence of substrate, shows a Soret band at 417 nm and alpha-,beta-, and delta bands at 569, 535, and 369 nm | Mycobacterium tuberculosis |