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Literature summary extracted from
- Construction of Escherichia coli BL21/A-53 producing histidine-tagged carboxymethylcellulase and comparison of its characteristics with CMCase without histidine-tag (2019), Prep. Biochem. Biotechnol., 49, 167-175 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.203 | Mn2+ | 5 mM, 39.6% residual activity | Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.203 | additional information | - |
(carboxymethyl)cellulose | Km value 9.3 g/l, pH 5.0, 50°C | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.203 | Co2+ | activating up to 100 mM | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.203 | Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.203 | 1718 | - |
pH 5.0, 50°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.203 | (carboxymethyl)cellulose + H2O | - |
Escherichia coli | (carboxymethyl)cellulose with endohydrolysed (1->4)-beta-D-glucosidic linkages | - |
? | |
| 3.2.1.203 | avicel + H2O | 29.8% of the activity with (carboxymethyl)cellulose | Escherichia coli | ? | - |
? | |
| 3.2.1.203 | filter paper + H2O | 19.5% of the activity with (carboxymethyl)cellulose | Escherichia coli | ? | - |
? | |
| 3.2.1.203 | additional information | no substrates: cellobiose, 4-nitrophenyl-beta-D-glucopyranoside | Escherichia coli | ? | - |
? | |
| 3.2.1.203 | pullulan + H2O | 7.2% of the activity with (carboxymethyl)cellulose | Escherichia coli | ? | - |
? | |
| 3.2.1.203 | xylan + H2O | 6.8% of the activity with (carboxymethyl)cellulose | Escherichia coli | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.203 | ? | x * 56260, calculated from sequence, His-tagged recombinant protein | Escherichia coli |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.203 | CMCase | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.203 | 50 | - |
- |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.203 | 5 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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