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Literature summary extracted from
- Purification and kinetics of a protease-resistant, neutral, and thermostable phytase from Bacillus subtilis subsp. subtilis JJBS250 ameliorating food nutrition (2018), Prep. Biochem. Biotechnol., 48, 718-724 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Triton X-100 | activates about 2fold at 1% | Bacillus subtilis subsp. subtilis |  |
| 3.1.3.8 | Tween 20 | activates about 2fold at 1% | Bacillus subtilis subsp. subtilis | |
| 3.1.3.8 | Tween 80 | activates about 2fold at 1% | Bacillus subtilis subsp. subtilis | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Al3+ | complete inhibition at 5 mM | Bacillus subtilis subsp. subtilis | |
| 3.1.3.8 | Fe2+ | complete inhibition at 5 mM | Bacillus subtilis subsp. subtilis | |
| 3.1.3.8 | Mg2+ | complete inhibition at 5 mM | Bacillus subtilis subsp. subtilis | |
| 3.1.3.8 | additional information | the purified enzyme is resistant to proteases trypsin and pepsin | Bacillus subtilis subsp. subtilis | |
| 3.1.3.8 | SDS | 30% inhibition at 1% | Bacillus subtilis subsp. subtilis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.293 | - |
myo-inositol hexakisphosphate | pH 7.0, 50°C, sodium phytate | Bacillus subtilis subsp. subtilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.8 | Ca2+ | stimulates 38% at 5 mM | Bacillus subtilis subsp. subtilis | |
| 3.1.3.8 | Co2+ | stimulates 70% at 5 mM | Bacillus subtilis subsp. subtilis | |
| 3.1.3.8 | K+ | stimulates 32% at 5 mM | Bacillus subtilis subsp. subtilis | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Bacillus subtilis subsp. subtilis | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | Bacillus subtilis subsp. subtilis JJBS250 | - |
1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Bacillus subtilis subsp. subtilis | O31097 | isolated from soil samples | - |
| 3.1.3.8 | Bacillus subtilis subsp. subtilis JJBS250 | O31097 | isolated from soil samples | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | native enzyme 35.87fold by ammonium sulfate fractionation, dialysis, anion exchange chromatography, lyophilization, and gel filtration | Bacillus subtilis subsp. subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 8.61 | - |
purified native enzyme, pH 7.0, 50°C | Bacillus subtilis subsp. subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | additional information | the purified enzyme shows specificity to different salts of phytic acid, it also shows phosphomonoesterase activity with ADP and ATP (high activity), while not with 4-nitrophenyl phosphate and glucose 6-phosphate | Bacillus subtilis subsp. subtilis | ? | - |
? | |
| 3.1.3.8 | additional information | the purified enzyme shows specificity to different salts of phytic acid, it also shows phosphomonoesterase activity with ADP and ATP (high activity), while not with 4-nitrophenyl phosphate and glucose 6-phosphate | Bacillus subtilis subsp. subtilis JJBS250 | ? | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis subsp. subtilis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | phytic acid, sodium phytate is preferred compared to calcium phytate and potassium phytate | Bacillus subtilis subsp. subtilis | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis subsp. subtilis JJBS250 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate + H2O | phytic acid, sodium phytate is preferred compared to calcium phytate and potassium phytate | Bacillus subtilis subsp. subtilis JJBS250 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | ? | x * 46000, SDS-PAGE | Bacillus subtilis subsp. subtilis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | PhyC | - |
Bacillus subtilis subsp. subtilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 70 | - |
- |
Bacillus subtilis subsp. subtilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 70 | - |
purified native enzyme, 10 min, about 75% activity is maintained | Bacillus subtilis subsp. subtilis |
| 3.1.3.8 | 80 | - |
purified native enzyme, 10 min, about 20% activity is maintained | Bacillus subtilis subsp. subtilis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 7 | - |
- |
Bacillus subtilis subsp. subtilis |
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