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Literature summary extracted from

  • Muench, D.; Roemer, T.; Lee, S.H.; Engeser, M.; Sahl, H.G.; Schneider, T.
    Identification and in vitro analysis of the GatD/MurT enzyme-complex catalyzing lipid II amidation in Staphylococcus aureus (2012), PLoS Pathog., 8, e1002509 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.13 expression in Escherichia coli Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.13 C94G mutation in subunit GaTD, abolishes lipid II amidation Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol Staphylococcus aureus
-
 ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol Staphylococcus aureus N315
-
 ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O Staphylococcus aureus
-
 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O Staphylococcus aureus N315
-
 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
6.3.5.13 beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3 Staphylococcus aureus
-
 beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
6.3.5.13 beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3 Staphylococcus aureus N315
-
 beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
6.3.5.13 L-glutamine + H2O Staphylococcus aureus
-
 L-glutamate + NH3
-
 ?
6.3.5.13 L-glutamine + H2O Staphylococcus aureus N315
-
 L-glutamate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.13 Staphylococcus aureus A0A0H3JN63 and A0A0H3JUU7 A0A0H3JN63 i.e subunit GatD, A0A0H3JUU7 i.e. subunit MurT
-
6.3.5.13 Staphylococcus aureus N315 A0A0H3JN63 and A0A0H3JUU7 A0A0H3JN63 i.e subunit GatD, A0A0H3JUU7 i.e. subunit MurT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.13 recombinant MurT/GatD complex Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol
-
 Staphylococcus aureus ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol
-
 Staphylococcus aureus N315 ADP + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol
-
 ?
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
6.3.5.13 ATP + beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + L-glutamine + H2O
-
 Staphylococcus aureus N315 ADP + phosphate + beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + L-glutamate
-
 ?
6.3.5.13 ATP + glutamate in lipid I + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + glutamine in lipid I + L-glutamate
-
 ?
6.3.5.13 ATP + glutamate in lipid II + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + glutamine in lipid II + L-glutamate
-
 ?
6.3.5.13 ATP + glutamate in lipid II-Gly5 + L-glutamine + H2O
-
 Staphylococcus aureus ADP + phosphate + glutamine in lipid II-Gly5 + L-glutamate
-
 ?
6.3.5.13 beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3
-
 Staphylococcus aureus beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
6.3.5.13 beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-O-P-Glu-L-Lys-D-Ala-D-Ala)-diphospho-ditrans,octacis-undecaprenol + NH3
-
 Staphylococcus aureus N315 beta-D-GlcNAc-(1->4)-MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenol + phosphate
-
 ?
6.3.5.13 L-glutamine + H2O
-
 Staphylococcus aureus L-glutamate + NH3
-
 ?
6.3.5.13 L-glutamine + H2O
-
 Staphylococcus aureus N315 L-glutamate + NH3
-
 ?
6.3.5.13 additional information both MurT and GatD are required for the reaction Staphylococcus aureus ?
-
 ?
6.3.5.13 additional information both MurT and GatD are required for the reaction Staphylococcus aureus N315 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.5.13 GatD/MurT
-
 Staphylococcus aureus
6.3.5.13 SA1707 subunit Gatd Staphylococcus aureus
6.3.5.13 SA1708 subunit Murt Staphylococcus aureus

General Information

EC Number General Information Comment Organism
6.3.5.13 physiological function enzyme complex catalyzes the amidation of the peptidoglycan complex, which is important for polymerization of cell wall building blocks. Inactivation of the GatD active site abolishes lipid II amidation Staphylococcus aureus