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Literature summary extracted from

  • Antonopoulou, I.; Hunt, C.; Cerullo, G.; Varriale, S.; Gerogianni, A.; Faraco, V.; Rova, U.; Christakopoulos, P.
    Tailoring the specificity of the type C feruloyl esterase FoFaeC from Fusarium oxysporum towards methyl sinapate by rational redesign based on small molecule docking simulations (2018), PLoS ONE, 13, e0198127 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.73 expression in Pichia pastoris Fusarium oxysporum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.73 homology modeling of structure and docking of substrates Fusarium oxysporum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.73 additional information introduction of mutations to provide hydrophobic environment for increased hydrolysis of sinapate substrates Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.331
-
 methyl ferulate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 0.424
-
 methyl sinapate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 0.649
-
 methyl caffeate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 0.799
-
 methyl p-coumarate wild-type, pH 6.0, 37°C Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Fusarium oxysporum A0A1D3S5H0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 methyl caffeate + H2O 85% of the activity with methyl p-coumarate Fusarium oxysporum methanol + caffeate
-
 ?
3.1.1.73 methyl ferulate + H2O 54% of the activity with methyl p-coumarate Fusarium oxysporum methanol + ferulate
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Fusarium oxysporum methanol + p-coumarate
-
 ?
3.1.1.73 methyl sinapate + H2O 2.8% of the activity with methyl p-coumarate Fusarium oxysporum methanol + sinapate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FaeC
-
 Fusarium oxysporum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 17
-
 methyl sinapate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 232
-
 methyl ferulate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 438
-
 methyl caffeate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 546
-
 methyl p-coumarate wild-type, pH 6.0, 37°C Fusarium oxysporum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.73 40
-
 methyl sinapate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 675
-
 methyl caffeate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 682
-
 methyl p-coumarate wild-type, pH 6.0, 37°C Fusarium oxysporum
3.1.1.73 744
-
 methyl ferulate wild-type, pH 6.0, 37°C Fusarium oxysporum